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Gene Expression Data
Assay Details
Assay
Reference: J:50582 Roebroek AJ, et al., Failure of ventral closure and axial rotation in embryos lacking the proprotein convertase Furin. Development. 1998 Dec;125(24):4863-76
Assay type: RT-PCR
MGI Accession ID: MGI:1329901
Gene symbol: Furin
Gene name: furin, paired basic amino acid cleaving enzyme
Probe: Pcsk3-pA, Pcsk3-pB
Visualized with: Ethidium bromide
Assay notes: Bottom panel shows KpnI digested PCR products. Digestion by KpnI is indicative of wild-type mRNA, and resistance to digestion suggests aberrant splicing events.
Results
Image: 2E ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 391 bp 300 bp Amount Genetic BackgroundMutant Allele(s)Sex
marker Control
+/+ E9.5 TS15: embryo Strong Weak Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Not Specified
+/+ E9.5 TS15: embryo Strong Weak Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Not Specified
+/- E9.5 TS15: embryo Strong Weak Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Furintm1Ajmr/Furin+Not Specified
+/- E9.5 TS15: embryo Strong Weak Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Furintm1Ajmr/Furin+Not Specified
-/- E9.5 TS15: embryo Moderate Moderate Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Furintm1Ajmr/Furintm1AjmrNot Specified
-/- E9.5 TS15: embryo Moderate Moderate Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Furintm1Ajmr/Furintm1AjmrNot Specified
2 PCR -/- E9.5 TS15: embryo Moderate Moderate Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Furintm1Ajmr/Furintm1AjmrNot Specified
2 PCR -/- E9.5 TS15: embryo Moderate Moderate Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Furintm1Ajmr/Furintm1AjmrNot Specified
no RT Control
fur cDNA Control
blanks Control
marker Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory