Gene Expression Data
Assay Details
Assay
|
Reference:
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J:43767
Lindahl P, et al., Alveogenesis failure in PDGF-A-deficient mice is coupled to lack of distal spreading of alveolar smooth muscle cell progenitors during lung development. Development. 1997 Oct;124(20):3943-53
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1330864
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Probe:
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PDGF-R alpha probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
|
It is possible that PDGF-R[alpha] probe3 (MGI:2448749) was used in this assay.
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|
Results
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Specimen
1B PDGF-Ra mRNA:
embryonic day 14.5
(more )
Specimen
1B PDGF-Ra mRNA:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lung mesenchyme |
Strong |
Regionally restricted |
1B
|
Expression was detected in clustered mesenchymal cells lining the distal epithelial tubules and terminal buds and was weak surrounding the proximal epithelium of prospective bronchi and branches.
|
Specimen
Not Shown:
embryonic day 15.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lung mesenchyme |
Strong |
Regionally restricted |
|
Expression was detected in clustered mesenchymal cells lining the distal epithelial tubules and terminal buds and was weak surrounding the proximal epithelium of prospective bronchi and branches.
|
Specimen
Not Shown:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lung mesenchyme |
Strong |
Regionally restricted |
|
Expression was detected in the mesenchyme surrounding distal epithelial terminal buds. Mutant was indistinguishable from wild type.
|
Specimen
2A:
embryonic day 15.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lung mesenchyme |
Strong |
Regionally restricted |
2A
|
Expression was detected in the mesenchyme surrounding distal epithelial terminal buds.
|
Specimen
2B:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lung mesenchyme |
Strong |
Regionally restricted |
2B
|
Expression was detected in the mesenchyme surrounding distal epithelial terminal buds. Mutant was indistinguishable from wild type.
|
Specimen
2C:
embryonic day 16.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lung mesenchyme |
Strong |
Regionally restricted |
2C
|
Expression was detected in the cells at the terminal buds. Cells were more dispersed than at E15.5.
|
Specimen
2D:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lung mesenchyme |
Strong |
Regionally restricted |
2D
|
Expression was detected in the distal epithelial terminal buds. In the mutant, there appeared to be fewer cells with no change in their distribution (essentially unchanged from that seen at E15.5) than seen in wild type.
|
Specimen
2E:
embryonic day 17.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung |
Present |
Single cells |
2E
|
Expression was detected in cells lining the lumen of terminal sacs and was strong in widely scattered numerous cells.
|
TS25: lung mesenchyme |
Weak |
Regionally restricted |
2E
|
Expression was detected in the cells surrounding the proximal epithelium and in the prospective bronchial and bronchiolar walls.
|
TS25: lung vascular element |
Weak |
Regionally restricted |
2E
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Expression was detected in the walls.
|
Specimen
2F:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung |
Present |
Regionally restricted |
2F
|
Expression was strong in clustered cells at the proximal tubule and was detected in thin walled tubules of the prospective terminal sacs but in hardly any cells compared to wild type.
|
Specimen
2G:
embryonic day 17.5
(more )
Specimen
2G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung |
Present |
Single cells |
2G
|
Expression was detected in cells lining the lumen of terminal sacs and was strong in widely scattered numerous cells.
|
TS25: lung mesenchyme |
Weak |
Regionally restricted |
2G
|
Expression was detected in the cells surrounding the proximal epithelium and in the prospective bronchial and bronchiolar walls.
|
TS25: lung vascular element |
Weak |
Regionally restricted |
2G
|
Expression was detected in the walls.
|
Specimen
2H:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung |
Present |
Regionally restricted |
2H
|
Expression was strong in clustered cells at the proximal tubule and was detected in thin walled tubules of the prospective terminal sacs but in hardly any cells compared to wild type.
|
Specimen
Not Shown:
embryonic day 18.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung |
Present |
Single cells |
|
Expression was detected in numerous cells in the walls of the terminal sacs.
|
Specimen
Not Shown:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung |
Absent |
|
|
There were no or very few positive cells in the walls of the terminal sacs.
|
Specimen
2I:
postnatal day 1
(more )
Specimen
2I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2J:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Not Shown:
postnatal day 4
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lung vascular element |
Present |
|
|
Expression was detected in the vascular walls.
|
TS28: alveolar wall |
Present |
|
|
Expression in numerous cells in the walls of the terminal sacs, but the number of cells declined from that seen at P1.
|
Specimen
Not Shown:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2K:
postnatal day 7
(more )
Specimen
2K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: alveolar wall |
Present |
|
2K
|
Expression was detected in numerous cells in the walls of the terminal sacs, but the number of cells declined from that seen at P1.
|
Specimen
2L:
(close )
Genetic Background:
|
involves: 129/Ola * 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Pdgfatm1Cbet/Pdgfatm1Cbet
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: alveolar wall |
Absent |
|
2L
|
Expression was absent from cells in the walls of the terminal sacs.
|
Specimen
4C:
postnatal day 4.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 4.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4D:
postnatal day 4.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 4.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Not Shown:
postnatal week 3
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal week 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|