Gene Expression Data
Assay Details
Assay
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Reference:
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J:50869
Ko MSH, et al., Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome. Hum Mol Genet. 1998 Nov;7(12):1967-78
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:1332145
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Gene symbol:
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Gtf2h5
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Gene name:
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general transcription factor IIH, polypeptide 5
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Probe:
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D17Wsu155e pA,pB
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Visualized with:
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Ethidium bromide
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Assay notes:
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Equal amounts of cDNA derived from extraembryonic tissues and embryonic tissues were sequentially diluted in 10-fold steps. Controls: the abundance of cytoskeletal beta-actin cDNA within extraembryonic tissue was shown to be the same as that in embryonic tissue; RNA samples without reverse transcriptase treatment did not show any PCR products.
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Results
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