Probe:	Pax-6 probe2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted		Pax-6 expression was confirmed to be in a longitudinal band through the alar plate from the region of the optic stalk to the border between the diencephalon and mesencephalon.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3A	Expression was restricted to the caudal part of the ventral thalamus, the dorsal midline of the dorsal thalamus and pretectum, with a mediolateral expansion on the dorsal surface of the caudal pretectum.
TS22: thalamus	Present	Regionally restricted	3A	Expression was restricted to the caudal part of the ventral thalamus, the dorsal midline of the dorsal thalamus and pretectum, with a mediolateral expansion on the dorsal surface of the caudal pretectum.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3B	Expression was restricted to the caudal part of the ventral thalamus, the dorsal midline of the dorsal thalamus and pretectum, with a mediolateral expansion on the dorsal surface of the caudal pretectum.
TS22: thalamus	Present	Regionally restricted	3B	Expression was restricted to the caudal part of the ventral thalamus, the dorsal midline of the dorsal thalamus and pretectum, with a mediolateral expansion on the dorsal surface of the caudal pretectum.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3C	Expression in the ventral thalamus was expanded and appeared to fill all of the ventral thalamus. Expression was detected along the dorsal midline of the dorsal thalamus and pretectum, although its caudal limit did not form an expanded and sharp boundary.
TS22: thalamus	Present	Regionally restricted	3C	Expression in the ventral thalamus was expanded and appeared to fill all of the ventral thalamus. Expression was detected along the dorsal midline of the dorsal thalamus and pretectum, although its caudal limit did not form an expanded and sharp boundary.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3D	Expression in the ventral thalamus was expanded and appeared to fill all of the ventral thalamus. Expression was detected along the dorsal midline of the dorsal thalamus and pretectum, although its caudal limit did not form an expanded and sharp boundary.
TS22: thalamus	Present	Regionally restricted	3D	Expression in the ventral thalamus was expanded and appeared to fill all of the ventral thalamus. Expression was detected along the dorsal midline of the dorsal thalamus and pretectum, although its caudal limit did not form an expanded and sharp boundary.