Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45322
Wong M, et al., Cyr61, product of a growth factor-inducible immediate-early gene, regulates chondrogenesis in mouse limb bud mesenchymal cells. Dev Biol. 1997 Dec 15;192(2):492-508
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1340255
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Gene symbol:
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Ccn1
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Gene name:
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cellular communication network factor 1
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Antibody:
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mAbIII
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1B:
embryonic day 13.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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NCD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1D:
embryonic day 13.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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NCD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1E:
embryonic day 13.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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NCD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb digit |
Present |
Regionally restricted |
1E
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Expression was found in the maturing cartilage and perichondrium of the digits in the forelimb. The most intense staining was in the perichondrium.
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| TS21: forelimb digit mesenchyme |
Present |
Regionally restricted |
1E
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Expression was found in the maturing cartilage and perichondrium of the digits in the forelimb. The most intense staining was in the perichondrium.
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| TS21: forelimb interdigital region mesenchyme |
Present |
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1E
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| TS21: forelimb |
Present |
Regionally restricted |
1E
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Expression staining was observed in all the cartilaginous anlages of the forelimb.
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Specimen
1F:
embryonic day 13.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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NCD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tail somite |
Present |
Regionally restricted |
1F
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Expression was localized in the less mature, condensing mesenchyme of the caudal somites as they differentiate into chrondrocytes.
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| TS21: axial skeleton |
Present |
Regionally restricted |
1F
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In the more mature cranial region, expression was detected in and around the differentiated vertebral cartilage.
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Analysis Tools