Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:35223
Coffin JD, et al., Angioblast differentiation and morphogenesis of the vascular endothelium in the mouse embryo. Dev Biol. 1991 Nov;148(1):51-62
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:1340549
|
|
Gene symbol:
|
Vwf
|
|
Gene name:
|
Von Willebrand factor
|
|
Antibody:
|
anti-VWF
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
|
Results
|
Specimen
5b:
embryonic day 7.0
(more  )
Note: Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
Specimen
5b:
(close  )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Absent |
|
5b
|
|
| TS28: decidua |
Present |
Regionally restricted |
5b
|
Endothelium in the decidua was generally positive for VWF.
|
Specimen
6b:
embryonic day 8.0
(more )
Note: Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
Specimen
6b:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: dorsal aorta |
Present |
Regionally restricted |
6b
|
VWF-positive endothelium appeared in the dorsal aortae and part of the endocardium.
|
| TS12: heart |
Present |
Regionally restricted |
6b
|
VWF-positive endothelium appeared in the dorsal aortae and part of the endocardium.
|
Specimen
7b:
embryonic day 10.0
(more )
Note: Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
Specimen
7b:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 10.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: dorsal aorta |
Present |
|
7b
|
|
| TS16: arterial system |
Present |
Regionally restricted |
7b
|
The intersomitic arteries were VWF-positive. The vertebral arteries that link with the intersomitic arteries in the circulatory pattern around the somites were VWF-negative.
|
| TS16: venous system |
Present |
Regionally restricted |
7b
|
On rare occasions, portions of the anterior cardinal veins and a few brain capillaries appeared immunopositive.
|
| TS16: anterior cardinal vein |
Present |
|
7b
|
On rare occasions, portions of the anterior cardinal veins and a few brain capillaries appeared immunopositive.
|
Specimen
8b,d:
embryonic day 12.0
(more )
Note: Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
Specimen
8b,d:
(close )
|
Genetic Background:
|
BALB/c
|
|
Age:
|
embryonic day 12.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Specimen was fixed on ice in PLP (0.01 M periodate, 0.01 M lysine, 2% paraformaldehyde).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cardiovascular system |
Present |
Regionally restricted |
8b
,
8d
|
VWF expression was limited to only the large vessel endothelium and possibly a few small vessels in close proximity to a large vessel.
|
|
Analysis Tools