Gene Expression Data
Assay Details
Assay
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Reference:
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J:40215
Wehrle-Haller B, et al., Soluble and cell-bound forms of steel factor activity play distinct roles in melanocyte precursor dispersal and survival on the lateral neural crest migration pathway. Development. 1995 Mar;121(3):731-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1341020
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Gene symbol:
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Dct
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Gene name:
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dopachrome tautomerase
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Probe:
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TRP-2 probe-3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1.B:
embryonic day 10.5
(more )
Specimen
1.B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1.D:
embryonic day 11.0
(more )
Specimen
1.D:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 11.0
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Sex:
|
Not Specified
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Type:
|
whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS18: head mesenchyme |
Present |
Regionally restricted |
1D
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Expressing cells were present in head mesenchyme particularly in the region between the eye, forebrain, and nose.
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TS18: branchial arch |
Present |
Regionally restricted |
1D
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Expression was detected in the posterior regions of the branchial arch.
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TS18: 2nd branchial arch |
Present |
Regionally restricted |
1D
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Expression was detected in the posterior regions of the hyoid arch.
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TS18: trunk mesenchyme |
Present |
Regionally restricted |
1D
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Expressing cells were observed from the first somites to the level of the hindlimb buds in the same locations as cells expressing Kit mRNA. No expression was detected in the lateral mesenchyme.
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TS18: forelimb bud |
Absent |
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1D
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TS18: hindgut |
Absent |
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1D
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TS18: hindlimb bud |
Absent |
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1D
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TS18: neural tube |
Absent |
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1D
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No expression was detected in the dorsal neural tube.
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Specimen
1.F:
embryonic day 11.0
(more )
Specimen
1.F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: head mesenchyme |
Present |
Regionally restricted |
1F
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Expressing cells were localized in a stripe originating at the mid-to-hindbrain junction and extending towards the eye. No expression was detected in facial structures.
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TS18: hindlimb bud |
Absent |
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1F
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Specimen
2.A:
embryonic day 11.5
(more )
Specimen
2.A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Specimen
2.B:
embryonic day 11.5
(more )
Specimen
2.B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: head mesenchyme |
Present |
Regionally restricted |
2B
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Expression was detected anterior and posterior to the eye, over the hindbrain, and around the otic vesicle.
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TS19: 2nd branchial arch |
Present |
Regionally restricted |
2B
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Expression was detected in the posterior aspect of the hyoid arch.
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Specimen
2.D:
embryonic day 11.5
(more )
Specimen
2.D:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS19: tail mesenchyme |
Present |
Regionally restricted |
2D
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Expressing cells were localized in the migrating staging area lateral to the neural tube, and just one or two somites more rostral cells were found over the somite lateral to the migrating staging area.
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Specimen
2.E:
embryonic day 11.5
(more )
Specimen
2.E:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: tail mesenchyme |
Present |
Regionally restricted |
2E
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In progressively older (more rostral) somites, incrementally more cells were localized laterally, and fewer positive cells remained near the neural tube.
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TS19: hindlimb bud |
Present |
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2E
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Authors report that expressing cells were first observed at the base of the hind limb bud.
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Specimen
3.A:
embryonic day 11.5
(more )
Specimen
3.A:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
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section from whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS19: tail mesenchyme |
Present |
Regionally restricted |
3A
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Two groups of positive cells were detected: one group in the migrating staging area lateral to the neural tube, and the other localized to the dermatome.
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Specimen
3.C:
embryonic day 11.5
(more )
Specimen
3.C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk mesenchyme |
Present |
Regionally restricted |
3C
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Expression was detected on the laminin-positive basement membrane.
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Specimen
3.E:
embryonic day 11.5
(more )
Specimen
3.E:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk mesenchyme |
Present |
Regionally restricted |
3E
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Expressing cells are evenly spread throughout the dermal mesenchyme at this axial level.
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Specimen
4.A:
embryonic day 12.5
(more )
Specimen
4.A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Present |
Regionally restricted |
4A
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Expression was detected in regions around the ear.
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TS20: trunk |
Present |
Regionally restricted |
4A
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Positive cells were detected between the neural tube and the lateral midline, and some cells as far ventral as the base of the forelimb bud.
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Specimen
4.B:
embryonic day 12.5
(more )
Specimen
4.B:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Present |
Regionally restricted |
4B
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Expression was detected in regions around the ear.
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TS20: trunk |
Present |
Regionally restricted |
4B
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Positive cells were detected between the neural tube and the lateral midline, and some cells as far ventral as the base of the forelimb bud.
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Specimen
4.C:
embryonic day 12.5
(more )
Specimen
4.C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Present |
Regionally restricted |
4C
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Expression was detected in regions around the ear, bordering the whisker fields, and over the nose.
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TS20: hindlimb |
Present |
Regionally restricted |
4C
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Many positive cells were present on the posterior side of the hindlimb.
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TS20: trunk |
Present |
Regionally restricted |
4C
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Positive cells were detected on the lateral sides of the tail.
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Specimen
4.D:
embryonic day 12.5
(more )
Specimen
4.D:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Present |
Regionally restricted |
4D
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Positive cells were detected between the neural tube and the lateral midline, and some cells as far ventral as the base of the forelimb bud.
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TS20: trunk |
Present |
Regionally restricted |
4D
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No expression was detected on the ventral side of the body.
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Specimen
6.A:
embryonic day 11.5
(more )
Specimen
6.A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
6.B:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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KitlSl/KitlSl
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: tail mesenchyme derived from neural crest |
Present |
Spotted |
6B
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Individual expressing cells were detected in the migrating staging area over a length of four to five segments.
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TS20: brain |
Present |
Spotted |
6B
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Expression was detected in a few cells at the mid-hindbrain border.
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Specimen
6.C:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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KitlSl-d/KitlSl-d
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS20: tail dermomyotome |
Present |
Spotted |
6C
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A lower density of expressing cells were localized over the dermatome compared to wild-type embryos.
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TS20: tail somite |
Present |
Spotted |
6C
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In contrast to Sl/Sl animals, many expressing cells were dispersed over the somites.
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TS20: brain |
Present |
Spotted |
6C
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Expression was detected in a few cells at the mid-hindbrain border.
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TS20: tail mesenchyme derived from neural crest |
Present |
Spotted |
6C
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Individual expressing cells were detected in the migrating staging area over a length of four to five segments.
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Specimen
6.D:
embryonic day 11.5; KitW/ KitW
(more )
Specimen
6.D:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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KitW/KitW
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: tail mesenchyme derived from neural crest |
Present |
Spotted |
6D
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Individual expressing cells were detected in the migrating staging area over a length of four to five segments. Expression pattern in the tail region was similar to the pattern in Sl/Sl mutants.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
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Type:
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whole mount
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