Prf1<tm1Sdz> Targeted Allele Detail MGI Mouse (MGI:1857235)

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Prf1^tm1Sdz
Targeted Allele Detail

Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Summary

Symbol:	Prf1^tm1Sdz
Name:	perforin 1 (pore forming protein); targeted mutation 1, Sandoz Pharmaceuticals
MGI ID:	MGI:1857235
Synonyms:	P⁰, perf-, perforin-, perforin 0, Pfn^-, pfp-, pfpKO, Pfp^tm1Sdz, pko, Prf^-, Prf1^-, prf1^tm1, Prf1^tm/Sdz
Gene:	Prf1 Location: Chr10:61133612-61140459 bp, + strand Genetic Position: Chr10, 32.18 cM
Alliance:	Prf1^tm1Sdz page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:17986
Parent Cell Line:	BL/6-III (ES Cell)
Strain of Origin:	C57BL/6

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutation:		Insertion
		Mutation details: A neomycin selection cassette was inserted into exon 3. RT-PCR analysis on RNA derived from homozygous mice demonstrated that an abnormal transcript was produced from this allele. However, immunocytochemistry experiments on activated spleen cells derived from homozygous mice confirmed that no detectable protein was made from this allele. (J:17986, J:96542)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 13 strains available Cell Lines: 0 lines available
Carrying any Prf1 Mutation:	51 strains or lines available

References

Original:	J:17986 Kagi D, et al., Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice [see comments]. Nature. 1994 May 5;369(6475):31-7
All:	362 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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