Ptgs1<tm1Unc> Targeted Allele Detail MGI Mouse (MGI:1857238)

Ptgs1^tm1Unc
Targeted Allele Detail

Summary

Symbol:	Ptgs1^tm1Unc
Name:	prostaglandin-endoperoxide synthase 1; targeted mutation 1, University of North Carolina
MGI ID:	MGI:1857238
Synonyms:	COX-1-, PGHS-1-, Ptgs1^-, Ptgs^tm1Smi
Gene:	Ptgs1 Location: Chr2:36120438-36142284 bp, + strand Genetic Position: Chr2, 24.19 cM
Alliance:	Ptgs1^tm1Unc page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:29511
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutations:		Insertion, Intragenic deletion
		Mutation details: A ~1 kb genomic fragment containing intron 10, and the first 44 bp of exon 11 was replaced with a neomycin selection cassette. Northern blot analysis on colon and kidney RNA demonstrated that a 1.1 kb aberrant transcript was produced from this allele. Western blot analysis on kidney, stomach or colon tissue lysates derived from homozygous mice confirmed that no detectable protein is produced from this allele, and additional western blots on proteins derived from LPS-induced macrophages derived from homozygous mice demonstrated that no encoded protein was present. (J:29511)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	8 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ptgs1^tm1Unc

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Ptgs1 Mutation:	46 strains or lines available

References

Original:	J:29511 Langenbach R, et al., Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration. Cell. 1995 Nov 3;83(3):483-92
All:	118 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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