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Lig4tm1Fwa
Targeted Allele Detail
Summary
Symbol: Lig4tm1Fwa
Name: ligase IV, DNA, ATP-dependent; targeted mutation 1, Frederick W Alt
MGI ID: MGI:1857732
Synonyms: Lig4-, LigIV-
Gene: Lig4  Location: Chr8:10020020-10027680 bp, - strand  Genetic Position: Chr8, 4.52 cM
Alliance: Lig4tm1Fwa page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:50865
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details: A neomycin resistance cassette replaced more than 50% of the coding sequences for the gene, removing the nucleotide sequences that encode amino acids 143 - 608 of the protein. (J:50865)
Phenotypes
Key:
hm homozygous ht heterozygous tg involves transgenes √ phenotype observed
cn conditional genotype  cx complex: > 1 genome feature ot other: hemizygous, indeterminate,... N normal phenotype
Genotype/
Background:
Allelic Composition
Genetic Background
Cell Line(s)
Allelic CompositionGenetic BackgroundCell Line(s)
hm1
Lig4tm1Fwa/Lig4tm1Fwa
involves: 129S6/SvEvTac
 
hm2
Lig4tm1Fwa/Lig4tm1Fwa
involves: 129S6/SvEvTac * C57BL/6
 
ht3
Lig4tm1Fwa/Lig4+
involves: 129S6/SvEvTac * C57BL/6
 
cn4
Lig4tm1Fwa/Lig4tm1Fwa
Tg(Cr2-cre)3Cgn/0
Xrcc4tm2Fwa/Xrcc4tm2Fwa
involves: 129S6/SvEvTac
 
cx5
Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Lig4tm1Fwa/Lig4tm1Fwa
Trp53tm1Tyj/Trp53tm1Tyj
involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac
 
cx6
Lig4tm1Fwa/Lig4tm1Fwa
Trp53tm1Brd/Trp53+
involves: 129S6/SvEvTac * 129S7/SvEvBrd
 
cx7
Lig4tm1Fwa/Lig4tm1Fwa
Trp53tm1Brd/Trp53tm1Brd
involves: 129S6/SvEvTac * 129S7/SvEvBrd
 
cx8
Xrcc5tm1Dbr/Xrcc5tm1Dbr
Lig4tm1Fwa/Lig4tm1Fwa
involves: 129S6/SvEvTac * C57BL/6
 
cx9
Xrcc5tm1Dbr/Xrcc5+
Lig4tm1Fwa/Lig4+
involves: 129S6/SvEvTac * C57BL/6
 
cx10
Paxxem1Spj/Paxxem1Spj
Lig4tm1Fwa/Lig4tm1Fwa
involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NTac
 
cx11
Cdkn2atm1Rdp/Cdkn2a+
Lig4tm1Fwa/Lig4tm1Fwa
involves: 129/Sv * 129S6/SvEvTac * C57BL/6J * SJL
 
cx12
Cdkn2atm1Rdp/Cdkn2atm1Rdp
Lig4tm1Fwa/Lig4tm1Fwa
involves: 129/Sv * 129S6/SvEvTac * C57BL/6J * SJL
 
Phenotypes:
Affected Systems
hm1
hm2
ht3
cn4
cx5
cx6
cx7
cx8
cx9
cx10
cx11
cx12
show or hide all annotated terms
                       
cellular
√ √ √ √ √ √ √ √ √ √
abnormal cell physiology
√ √
increased cellular sensitivity to ionizing radiation
√ √ √ √
increased cellular sensitivity to gamma-irradiation
√
decreased neuron apoptosis
√ √
increased neuron apoptosis
√
increased forebrain apoptosis
√
increased spinal cord apoptosis
√
increased fibroblast proliferation
√
decreased cell proliferation
√ √ √
chromosomal instability
√
chromosome breakage
√ √ √ √
induced chromosome breakage
√
spontaneous chromosome breakage
√
digestive/alimentary system
√
abnormal small intestine morphology
√
endocrine/exocrine glands
√ √
small thymus
√
decreased thymocyte number
√ √
growth/size/body
√ √ √
decreased body weight
√ √
decreased fetal size
√
hematopoietic system
√ √ √ √ √
hematopoietic system phenotype
N
small thymus
√
abnormal lymphocyte morphology
√
decreased thymocyte number
√ √
abnormal lymphopoiesis
√
abnormal B cell differentiation
√
abnormal class switch recombination
√ √
arrested B cell differentiation
√
abnormal T cell differentiation
√
arrested T cell differentiation
√
increased B cell number
√
immune system
√ √ √ √ √
small thymus
√
abnormal lymphocyte morphology
√
decreased thymocyte number
√ √
abnormal lymphopoiesis
√
abnormal B cell differentiation
√
abnormal class switch recombination
√ √
arrested B cell differentiation
√
abnormal T cell differentiation
√
arrested T cell differentiation
√
increased B cell number
√
mortality/aging
√ √ √ √ √ √ √
mortality/aging
N
premature death
√ √
lethality throughout fetal growth and development, incomplete penetrance
√
prenatal lethality, complete penetrance
√ √ √ √
prenatal lethality, incomplete penetrance
√ √ √
neoplasm
√
increased lymphoma incidence
√
nervous system
√ √ √ √
decreased neuron apoptosis
√ √
increased neuron apoptosis
√
increased forebrain apoptosis
√
increased spinal cord apoptosis
√
abnormal brainstem morphology
√
abnormal striatum morphology
√
abnormal diencephalon morphology
√
thin cerebral cortex
√
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 7 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying Lig4tm1Fwa
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Lig4 Mutation:  46 strains or lines available
References
Original:  J:50865 Frank KM, et al., Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV. Nature. 1998 Nov 12;396(6707):173-7
All:  32 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory