About   Help   FAQ
Lig4tm1Fwa
Targeted Allele Detail
Summary
Symbol: Lig4tm1Fwa
Name: ligase IV, DNA, ATP-dependent; targeted mutation 1, Frederick W Alt
MGI ID: MGI:1857732
Synonyms: Lig4-, LigIV-
Gene: Lig4  Location: Chr8:10020020-10027680 bp, - strand  Genetic Position: Chr8, 4.52 cM
Alliance: Lig4tm1Fwa page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:50865
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neomycin resistance cassette replaced more than 50% of the coding sequences for the gene, removing the nucleotide sequences that encode amino acids 143 - 608 of the protein. (J:50865)
Phenotypes
Key:
hm homozygous ht heterozygous tg involves transgenes phenotype observed
cn conditional genotype  cx complex: > 1 genome feature ot other: hemizygous, indeterminate,... N normal phenotype
Genotype/
Background:
Allelic Composition
Genetic Background
Cell Line(s)
Allelic CompositionGenetic BackgroundCell Line(s)
involves: 129S6/SvEvTac
 
involves: 129S6/SvEvTac * C57BL/6
 
Lig4tm1Fwa/Lig4+
involves: 129S6/SvEvTac * C57BL/6
 
involves: 129S6/SvEvTac
 
involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac
 
involves: 129S6/SvEvTac * 129S7/SvEvBrd
 
involves: 129S6/SvEvTac * 129S7/SvEvBrd
 
involves: 129S6/SvEvTac * C57BL/6
 
Xrcc5tm1Dbr/Xrcc5+
Lig4tm1Fwa/Lig4+
involves: 129S6/SvEvTac * C57BL/6
 
involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NTac
 
involves: 129/Sv * 129S6/SvEvTac * C57BL/6J * SJL
 
involves: 129/Sv * 129S6/SvEvTac * C57BL/6J * SJL
 
Phenotypes:
Affected Systems
show or hide all annotated terms
                       
cellular
abnormal cell physiology
increased cellular sensitivity to ionizing radiation
increased cellular sensitivity to gamma-irradiation
decreased neuron apoptosis
increased neuron apoptosis
increased forebrain apoptosis
increased spinal cord apoptosis
increased fibroblast proliferation
decreased cell proliferation
chromosomal instability
chromosome breakage
induced chromosome breakage
spontaneous chromosome breakage
digestive/alimentary system
abnormal small intestine morphology
endocrine/exocrine glands
small thymus
decreased thymocyte number
growth/size/body
decreased body weight
decreased fetal size
hematopoietic system
hematopoietic system phenotype
N
small thymus
abnormal lymphocyte morphology
decreased thymocyte number
abnormal lymphopoiesis
abnormal B cell differentiation
abnormal class switch recombination
arrested B cell differentiation
abnormal T cell differentiation
arrested T cell differentiation
increased B cell number
immune system
small thymus
abnormal lymphocyte morphology
decreased thymocyte number
abnormal lymphopoiesis
abnormal B cell differentiation
abnormal class switch recombination
arrested B cell differentiation
abnormal T cell differentiation
arrested T cell differentiation
increased B cell number
mortality/aging
mortality/aging
N
premature death
lethality throughout fetal growth and development, incomplete penetrance
prenatal lethality, complete penetrance
prenatal lethality, incomplete penetrance
neoplasm
increased lymphoma incidence
nervous system
decreased neuron apoptosis
increased neuron apoptosis
increased forebrain apoptosis
increased spinal cord apoptosis
abnormal brainstem morphology
abnormal striatum morphology
abnormal diencephalon morphology
thin cerebral cortex
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 7 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying Lig4tm1Fwa
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Lig4 Mutation:  46 strains or lines available
References
Original:  J:50865 Frank KM, et al., Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV. Nature. 1998 Nov 12;396(6707):173-7
All:  32 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
12/10/2024
MGI 6.24
The Jackson Laboratory