Gli1tm1Alj
Targeted Allele Detail
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| Symbol: |
Gli1tm1Alj |
| Name: |
GLI-Kruppel family member GLI1; targeted mutation 1, Alexandra L Joyner |
| MGI ID: |
MGI:1857797 |
| Synonyms: |
Gli1-, Gli1zfd |
| Gene: |
Gli1 Location: Chr10:127165751-127177448 bp, - strand Genetic Position: Chr10, 74.5 cM
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| Alliance: |
Gli1tm1Alj page
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A neomycin resistance cassette replaced exons 9 to 11, which encode part of zinc finger 1, zinc fingers 2 - 5, and 43 adjacent amino acids. Northern blots of total RNA from E13.5 embryos using a cDNA probe 3' to the zinc fingers resulted in detection of 2 transcripts in wild-type embryos and only one smaller broad band in homozygous mutant embryos. Further analysis by RT-PCR followed by sequencing of the products showed that the mutant allele produced a transcript that spliced around the neomycin insert from exon 8 - 12. The authors state that as a result, the mutant allele could not encode for a protein with DNA-binding activity, but could produce a mutant protein lacking amino acids 269 - 437.
(J:60986)
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View phenotypes and curated references for all genotypes (concatenated display).
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List all tumor models in MMHCdb carrying
Gli1tm1Alj
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Gli1 Mutation: |
49 strains or lines available
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| Original: |
J:60986 Park HL, et al., Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation. Development. 2000 Apr;127(8):1593-605 |
| All: |
28 reference(s) |
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Analysis Tools
