Gene Expression Data
Assay Details
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Assay
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Reference:
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J:19631
McKay IJ, et al., The kreisler mouse: a hindbrain segmentation mutant that lacks two rhombomeres. Development. 1994 Aug;120(8):2199-211
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1932929
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Gene symbol:
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Hoxd4
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Gene name:
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homeobox D4
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Probe:
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Hox-5.1 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Kreisler -/- mutants at dpc 9.5 have an absence of morphological segmentation posterior to the boundary between r3 and r4.
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Results
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Specimen
2 J:
embryonic day 9.5; Mafbkr/Mafb +
(more  )
Specimen
2 J:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafb+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Specimen
2 K:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 5 |
Present |
Graded |
2K
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The graded expression normally seen in r7 is now seen in the region that would normally be r5.
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| TS15: future hindbrain |
Present |
Regionally restricted |
2K
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The anterior limit of expression appears almost level with the caudal edge of the otocyst, anterior to its normal location.
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Analysis Tools