Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:45301
Cygan JA, et al., Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants. Development. 1997 Dec;124(24):5021-32
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1933251
|
Gene symbol:
|
Lmx1b
|
Gene name:
|
LIM homeobox transcription factor 1 beta
|
Probe:
|
Lmx1b cDNA1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1A:
embryonic day 8.5
(more )
Note: 10 somites.
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
10 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: lateral plate mesoderm |
Present |
Regionally restricted |
1A
|
Expression in the lateral plate mesoderm of the presumptive forelimb level.
|
Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
1C
|
Expression in cells approximately 9-12 cell layers beneath the dorsal ectoderm.
|
Specimen
1D:
embryonic day 13.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1E:
embryonic day 14.5
(more )
Specimen
1E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forearm |
Present |
|
1E
|
Expression levels have dropped.
|
TS22: hand mesenchyme |
Strong |
Regionally restricted |
1E
|
Expression in the dorsal mesenchyme of the autopod.
|
Specimen
1F:
embryonic day 14.5
(more )
Specimen
1F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2A:
embryonic day 11.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
2A
|
Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
|
TS19: handplate mesenchyme |
Present |
Regionally restricted |
2A
|
Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
|
Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
2B
|
Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
|
TS19: handplate mesenchyme |
Present |
Regionally restricted |
2B
|
Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
|
Specimen
2C:
(close )
Genetic Background:
|
involves: 129
|
Mutant Allele(s):
|
Wnt7atm1Amc/Wnt7atm1Amc
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: handplate mesenchyme |
Present |
Regionally restricted |
2C
|
Up to 11.5 dpc expression is the same as in wild-type. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud. The anterior mesenchyme is more severely affected.
|
Specimen
2D:
(close )
Genetic Background:
|
involves: 129
|
Mutant Allele(s):
|
Wnt7atm1Amc/Wnt7atm1Amc
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: handplate mesenchyme |
Present |
Regionally restricted |
2D
|
Up to 11.5 dpc expression is the same as in wild-type. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud.
|
Specimen
2E:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
2F
|
Expression is similar to wild-type in the dorsal mesenchyme and an additional ectopic domain in the ventral mesenchyme.
|
TS19: handplate mesenchyme |
Present |
Regionally restricted |
2F
|
Expression is similar to wild-type in the dorsal mesenchyme and an additional ectopic domain in the ventral mesenchyme.
|
Specimen
2G:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj Wnt7atm1Amc/Wnt7atm1Amc
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
2G
|
Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud. The anterior mesenchyme is more severely affected.
|
TS19: handplate mesenchyme |
Present |
Regionally restricted |
2G
|
Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud. The anterior mesenchyme is more severely affected.
|
Specimen
2H:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj Wnt7atm1Amc/Wnt7atm1Amc
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
2H
|
Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. No expression in the ventral mesenchyme.
|
TS19: handplate mesenchyme |
Present |
Regionally restricted |
2H
|
Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. No expression in the ventral mesenchyme.
|
Specimen
3A:
embryonic day 14.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hand mesenchyme |
Present |
Regionally restricted |
3A
|
Expression in the dorsal mesenchyme of the autopod.
|
Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hand mesenchyme |
Present |
Regionally restricted |
3C
|
Expression in the dorsal mesenchyme of the autopod.
|
Specimen
3E:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3G:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3I:
(close )
Genetic Background:
|
involves: 129
|
Mutant Allele(s):
|
Wnt7atm1Amc/Wnt7atm1Amc
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3K:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj Wnt7atm1Amc/Wnt7atm1Amc
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5K:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Spotted |
5K
|
Patchy expression in the ventral limb bud.
|
Specimen
5L:
embryonic day 11.5
(more )
Note: Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
Specimen
5L:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
5L
|
Expression in the dorsal mesenchyme terminates at the level of Fgf8 expression in the ectoderm.
|
TS19: handplate mesenchyme |
Present |
Regionally restricted |
5L
|
Expression in the dorsal mesenchyme terminates at the level of Fgf8 expression in the ectoderm.
|
Specimen
5M:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
En1tm1Alj/En1tm1Alj
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future arm mesenchyme |
Present |
Regionally restricted |
5M
|
A similar correlation between the distal extent of ectopic Lmx1b expression in the ventral mesenchyme and the position of ectopic Fgf8 expression in the ventral ectoderm.
|
TS19: handplate mesenchyme |
Present |
Regionally restricted |
5M
|
A similar correlation between the distal extent of ectopic Lmx1b expression in the ventral mesenchyme and the position of ectopic Fgf8 expression in the ventral ectoderm.
|
|