Prl<tm1Hmn> Targeted Allele Detail MGI Mouse (MGI:1934021)

Prl^tm1Hmn
Targeted Allele Detail

Summary

Symbol:	Prl^tm1Hmn
Name:	prolactin; targeted mutation 1, Nelson D Horseman
MGI ID:	MGI:1934021
Synonyms:	PRL-
Gene:	Prl Location: Chr13:27241553-27249187 bp, + strand Genetic Position: Chr13, 12.25 cM
Alliance:	Prl^tm1Hmn page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:45243
Parent Cell Line:	D3 (ES Cell)
Strain of Origin:	129S2/SvPas

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutation:		Insertion
		Mutation details: A PGK neomycin cassette was inserted into exon 4. This sequence encodes the second alpha helix of the protein. RT-PCR analysis confirmed a lack of wild-type transcript in homozygous mutant mice. Western blotting of pituitary gland extracts revealed the presence of a unique immunoreactive ~10-kDa truncated protein product in homozygous mutant mice. Disrupted prolactin synthesis was confirmed by biological assays. (J:45243)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

Tumor Data

List all tumor models in MMHCdb carrying Prl^tm1Hmn

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 4 strains available Cell Lines: 0 lines available
Carrying any Prl Mutation:	28 strains or lines available

References

Original:	J:45243 Horseman ND, et al., Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene. EMBO J. 1997 Dec 1;16(23):6926-35
All:	28 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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