Gene Expression Data
Assay Details
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Assay
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Reference:
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J:29332
Baldwin HS, et al., Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994 Sep;120(9):2539-53
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2136884
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Probe:
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Pecam-pG, Pecam-pH
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Visualized with:
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Ethidium bromide
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Assay notes:
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Outside primers were used first to amplify a region between position 116 and 688 (lanes 2-5) which was not detected, then the inside primers were used on the same sample to amplify a 383 bp fragment (see below lanes 7-9 and 11-13).
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Results
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| Mol. Wt. Marker Lane |
| Control |
| Control |
| Control |
| Control |
| Control |
| E7.5
(a)
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TS11: embryo
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Present
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Absent
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Absent
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Not Specified; poly-A+ RNA |
CD-1 |
| Not Specified |
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| E8.0
(b)
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TS12: embryo
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Present
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Absent
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Absent
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Not Specified; poly-A+ RNA |
CD-1 |
| Not Specified |
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| E8.5
(c)
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TS13: embryo
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Present
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Absent
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Absent
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Not Specified; poly-A+ RNA |
CD-1 |
| Not Specified |
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| Control |
| E7.5
(a)
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TS11: embryo
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Absent
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Present
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Present
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Not Specified; poly-A+ RNA |
CD-1 |
| Not Specified |
RT-PCR products were cut with DraI (position 559) which generated 2 bands 254 and 129 bp).
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| E8.0
(b)
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TS12: embryo
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Absent
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Present
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Present
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Not Specified; poly-A+ RNA |
CD-1 |
| Not Specified |
RT-PCR products were cut with DraI (position 559) which generated 2 bands 254 and 129 bp).
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| E8.5
(c)
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TS13: embryo
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Absent
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Present
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Present
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Not Specified; poly-A+ RNA |
CD-1 |
| Not Specified |
RT-PCR products were cut with DraI (position 559) which generated 2 bands 254 and 129 bp).
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Notes:
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Analysis Tools