Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:21621
Chang DT, et al., Products, genetic linkage and limb patterning activity of a murine hedgehog gene. Development. 1994 Nov;120(11):3339-53
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:2137954
|
Gene symbol:
|
Shh
|
Gene name:
|
sonic hedgehog
|
Probe:
|
pD7c8.1
|
Probe preparation:
|
Antisense labelled with P33 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
3B:
embryonic day 7.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Present |
Regionally restricted |
3B
|
Present at the anterior midline in the axial mesoderm. No expression was seen in other embryonic tissues.
|
TS11: embryo ectoderm |
Absent |
|
3B
|
Absent from the ectoderm overlying the anterior midline mesoderm.
|
Specimen
3C:
embryonic day 7.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Present |
Regionally restricted |
3C
|
Present at the anterior midline in the axial mesoderm. No expression was seen in other embryonic tissues.
|
TS11: embryo ectoderm |
Absent |
|
3C
|
Absent from the anterior midline ectoderm.
|
Specimen
Not shown:
embryonic day 7.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3D:
embryonic day 7.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Present |
Regionally restricted |
3D
|
Present at the anterior midline in the axial mesoderm.
|
Specimen
3E:
embryonic day 7.5
(more )
Specimen
3E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Present |
Regionally restricted |
3E
|
Present at the anterior midline in the axial mesoderm.
|
Specimen
3F:
embryonic day 7.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Present |
Regionally restricted |
3F
|
Present at the anterior midline in the axial mesoderm.
|
Specimen
Not shown:
embryonic day 8.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo mesenchyme |
Present |
Regionally restricted |
|
Present at the anterior midline in the axial mesoderm.
|
Specimen
4A,B:
embryonic day 9.5
(more )
Specimen
4A,B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future midbrain |
Strong |
Regionally restricted |
4A/B
|
The expression encompasses 40 percent of the ventral neural tube at its maximum extent in the midbrain. Also present in a ring of cells in the ventral surface of the midbrain-diencephalic region.
|
TS15: diencephalon |
Present |
Regionally restricted |
4A/B
|
Present in a small patch in the ventral diencephalon at the midline.
|
Specimen
4C:
embryonic day 9.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Strong |
Regionally restricted |
4C
|
Present at the ventral midline of the neural tube caudal to the hindbrain.
|
TS15: diencephalon |
Present |
Regionally restricted |
4C
|
Present in the ventral diencephalon and in a ring of cells in the ventral surface of the midbrain-diencephalic region.
|
TS15: future forebrain |
Strong |
Regionally restricted |
4C
|
Present in the ventral boundary region of the midbrain/forebrain.
|
TS15: telencephalon |
Present |
|
4C
|
The author states that expression is present rostral to the midbrain.T
|
Specimen
4D:
embryonic day 9.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Strong |
Regionally restricted |
4D
|
Present at the ventral midline of the neural tube caudal to the hindbrain. This includes cells adjacent to the optic vesicle.
|
TS15: diencephalon |
Present |
|
4D
|
The authors state that expression is present rostral to the midbrain but that it splits and extends bilaterally.
|
TS15: telencephalon |
Present |
|
4D
|
The authors state that expression is present rostral to the midbrain but that it splits and extends bilaterally.
|
Specimen
4E:
embryonic day 9.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Strong |
Regionally restricted |
4E
|
Present at the ventral midline of the neural tube caudal to the hindbrain. This includes ventral neural tube cells overlying the pharngeal lumen and in cells adjacent to the optic vesicle.
|
TS15: diencephalon |
Present |
Regionally restricted |
4E
|
Present in the ventral diencephalon and in a ring of cells in the ventral surface of the midbrain-diencephalic region. The author states that expression rostral to the midbrain splits and extends bilaterally, reuniting in the ventral midline.
|
TS15: telencephalon |
Present |
Regionally restricted |
4E
|
The authors state that expression is present rostral to the midbrain but that it splits and extends bilaterally, reuniting in the ventral midline at the diencephalon.
|
Specimen
4F:
embryonic day 9.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4G,H:
embryonic day 9.5
(more )
Specimen
4G,H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4I:
embryonic day 9.5
(more )
Specimen
4I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5A,B:
embryonic day 10.5
(more )
Specimen
5A,B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5C:
embryonic day 10.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5D:
embryonic day 10.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5E:
embryonic day 10.5
(more )
Specimen
5E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trachea |
Present |
Regionally restricted |
5E
|
Present in the epithelia of the tracheal lumen.
|
Specimen
5F:
embryonic day 10.5
(more )
Specimen
5F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5H:
embryonic day 10.5
(more )
Specimen
5H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5I:
embryonic day 10.5
(more )
Specimen
5I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5J:
embryonic day 10.5
(more )
Specimen
5J:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Not shown:
embryonic day 10.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|