Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:45780
Tiret L, et al., Increased apoptosis of motoneurons and altered somatotopic maps in the brachial spinal cord of Hoxc-8-deficient mice. Development. 1998 Jan;125(2):279-91
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:2149089
|
Gene symbol:
|
Isl1
|
Gene name:
|
ISL1 transcription factor, LIM/homeodomain
|
Antibody:
|
anti-C-Isl-1 protein (K5)
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
Assay notes:
|
Antibody used detects both Isl1 and Isl2; both genes have been annotated.
|
|
Results
|
Specimen
2F:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
2F:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Regionally restricted |
2F
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
3I - C6:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3I
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
|
Specimen
3J - C7:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3J
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
|
Specimen
3K - C8 LMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3K
|
Expression was detected in motor neurons of the lateral motor column. The specimen was from the level of the cervical vertebra 8.
|
Specimen
3L - C8 MMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3L
|
Expression was detected in motor neurons of the medial motor column. The specimen was from the level of the cervical vertebra 8.
|
Specimen
3M - C6:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3M - C6:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3M
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
|
Specimen
3N - C7:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3N - C7:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3N
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
|
Specimen
3O - C8 LMC:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3O - C8 LMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3O
|
Expression was detected in motor neurons of the lateral motor column. The specimen was from the level of the cervical vertebra 8.
|
Specimen
3P - C8 MMC:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3P - C8 MMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3P
|
Expression was detected in motor neurons of the medial motor column. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4A:
embryonic day 12.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
4A:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Single cells |
4A
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4B:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Single cells |
4B
|
Expression was detected in motor neurons, and the number did not differ from the heterozygous embryo. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4M:
embryonic day 15.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
4M:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: spinal cord |
Present |
Single cells |
4M
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4N:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: spinal cord |
Present |
Single cells |
4N
|
Expression was detected in motor neurons, but there were fewer motor neurons in the mutant. The specimen was from the level of the cervical vertebra 8.
|
|