Ephb2tm2Paw
Targeted Allele Detail
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| Symbol: |
Ephb2tm2Paw |
| Name: |
Eph receptor B2; targeted mutation 2, Tony Pawson |
| MGI ID: |
MGI:2149670 |
| Synonyms: |
EphB2ki, EphB2lacZ, EphB2lacZ(kin-), EphB2/LZ, EphB2N2, NuklacZ |
| Gene: |
Ephb2 Location: Chr4:136374850-136563299 bp, - strand Genetic Position: Chr4, 69.0 cM, cytoband D-E
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| Alliance: |
Ephb2tm2Paw page
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutation: |
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Insertion
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Mutation details: Insertion of a LacZ-neomycin resistance cassette in-frame at codon 622. Western blot analysis using a polyclonal Ephb2 antibody did not detect protein in cell surface immunoprecipitates of primary brain cultures from homozygous mutant neonates (P4), however a polyclonal beta-galactosidase antibody did detect a novel 200 kDa protein in these cultures. The authors propose that the mutant allele produces a fusion receptor protein comprised of the extracellular, transmembrane, and juxtamembrane domains (amino acids 1-621) linked to beta-galactosidase, and that this mutated receptor lacks the entire tyrosine kinase catalytic and C-terminal domains (amino acids 622-707).
(J:34200)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Ephb2 Mutation: |
68 strains or lines available
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| Original: |
J:34200 Henkemeyer M, et al., Nuk controls pathfinding of commissural axons in the mammalian central nervous system. Cell. 1996 Jul 12;86(1):35-46 |
| All: |
49 reference(s) |
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Analysis Tools
