Nos3<tm1Plh> Targeted Allele Detail MGI Mouse (MGI:2150145)

Nos3^tm1Plh
Targeted Allele Detail

Summary

Symbol:	Nos3^tm1Plh
Name:	nitric oxide synthase 3, endothelial cell; targeted mutation 1, Paul L Huang
MGI ID:	MGI:2150145
Synonyms:	eNOS^-, eNOS KO, NOSIII^-
Gene:	Nos3 Location: Chr5:24569808-24589472 bp, + strand Genetic Position: Chr5, 11.32 cM
Alliance:	Nos3^tm1Plh page

Renal tubular apoptosis, regeneration, glomerulosclerosis and glomerular thrombus formation in Nos1^tm1Plh/Nos1^tm1Plh Nos2^tm1Mrl/Nos2^tm1Mrl Nos3^tm1Plh/Nos3^tm1Plh mice

Show the 1 phenotype image(s) involving this allele.

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:28778
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutations:		Insertion, Intragenic deletion
		Mutation details: A neomycin cassette replaced 3 exons that encode the NADPH ribose and adenine binding sites of the protein (amino acids 1010 - 1144). Western blots of protein extracts of brain, heart, lung, and aorta/vena cava from homozygous mutant mice showed no detectable encoded protein. (J:28778)
Inheritance:		Dominant

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	28 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Nos3 Mutation:	58 strains or lines available

References

Original:	J:28778 Huang PL, et al., Hypertension in mice lacking the gene for endothelial nitric oxide synthase [see comments]. Nature. 1995 Sep 21;377(6546):239-42
All:	128 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 12/10/2024 MGI 6.24