Apptm1Cwe
Targeted Allele Detail
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| Symbol: |
Apptm1Cwe |
| Name: |
amyloid beta precursor protein; targeted mutation 1, Charles Weissmann |
| MGI ID: |
MGI:2154522 |
| Synonyms: |
beta APP delta |
| Gene: |
App Location: Chr16:84751236-84972187 bp, - strand Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
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| Alliance: |
Apptm1Cwe page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:21937
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| Parent Cell Line: |
D3 (ES Cell)
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| Strain of Origin: |
129S2/SvPas
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutation: |
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Insertion
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Mutation details: A neomycin resistance cassette was inserted into exon 2 of the gene. Northern blots of brain, kidney, and lung tissues from homozygous mutant mice showed no detectable full-length wild-type mRNA. Two smaller bands were detectable by Northern blot. The authors attributed these bands to transcription through the inserted neomycin cassette and beyond. Further analysis by Western blot of brain extracts from homozygous mutant mice showed no full-length wild-type protein. Again, two bands, which the authors attributed to carboxy-terminal breakdown products of the shortened protein, were seen. The resulting proteins were determined to lack amino acids 20 - 75.
(J:21937)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any App Mutation: |
105 strains or lines available
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| Original: |
J:21937 Muller U, et al., Behavioral and anatomical deficits in mice homozygous for a modified beta-amyloid precursor protein gene. Cell. 1994 Dec 2;79(5):755-65 |
| All: |
14 reference(s) |
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Analysis Tools
