Trp53tm1Holl
Targeted Allele Detail
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| Symbol: |
Trp53tm1Holl |
| Name: |
transformation related protein 53; targeted mutation 1, Monica Hollstein |
| MGI ID: |
MGI:2157167 |
| Synonyms: |
HUPKI, Hupki R72, p5372arg, p53hki, p53KI |
| Gene: |
Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
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| Alliance: |
Trp53tm1Holl page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:68918
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| Parent Cell Line: |
E14.1 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Humanized sequence) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Insertion of a loxP-flanked neomycin-thymidine kinase expression cassette replaced the mouse DNA-binding domain (exons 4-9) with the corresponding homologous segment of the normal human gene, leaving transcription under control of the endogenous promoter. Cre-mediated recombination in ES cells excised the neo-tk sequences. cDNA sequencing studies determined that transcripts from spleen of homozygous mutant mice carried the human sequence for exons 4-9, and that the gene is correctly spliced and transcribed.
(J:68918)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:68918 Luo JL, et al., Knock-in mice with a chimeric human/murine p53 gene develop normally and show wild-type p53 responses to DNA damaging agents: a new biomedical research tool. Oncogene. 2001 Jan 18;20(3):320-8 |
| All: |
18 reference(s) |
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Analysis Tools
