Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Tumor data | Find Mice (IMSR) | References

Symbol:	Akt2tm1.1Mbb
Name:	thymoma viral proto-oncogene 2; targeted mutation 1.1, Morris J Birnbaum
MGI ID:	MGI:2158456
Synonyms:	Akt2-, Akt2 KO, PKBbeta-
Gene:	Akt2  Location: Chr7:27290977-27340251 bp, + strand  Genetic Position: Chr7, 15.94 cM, cytoband B1
Alliance:	Akt2tm1.1Mbb page

Germline Transmission:	Earliest citation of germline transmission: J:71491
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: This allele is a derivative of Akt2tm1Mbb. Cre-mediated recombination in vivo under the control of a 6 kb 5'-flanking sequence from the Pou3f4 gene excised the floxed exons 4 and 5 in the germline. The excision of exons 4 and 5 results in a frameshift mutation that would lead to a premature termination even if the remaining exon 3 were to splice to exon 6. Exon 5 encodes the lysine residue necessary for catalytic activity. Western blot analyses using a polyclonal antibody did not detect protein in liver, muscle, and isolated adipocytes from homozygous mice. (J:71491)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

List all tumor models in MMHCdb carrying Akt2tm1.1Mbb

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Akt2 Mutation:	53 strains or lines available

Original:	J:71491 Cho H, et al., Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science. 2001 Jun 1;292(5522):1728-31
All:	103 reference(s)