Bdnf<tm3Jae> Targeted Allele Detail MGI Mouse (MGI:2176750)

Bdnf^tm3Jae
Targeted Allele Detail

Summary

Symbol:	Bdnf^tm3Jae
Name:	brain derived neurotrophic factor; targeted mutation 3, Rudolf Jaenisch
MGI ID:	MGI:2176750
Synonyms:	Bdnf², Bdnf^2L, Bdnf^2lox, Bdn^flox, Bdnf^tm2Jae
Gene:	Bdnf Location: Chr2:109505045-109557352 bp, + strand Genetic Position: Chr2, 56.63 cM
Alliance:	Bdnf^tm3Jae page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:71969
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Mutation
description

Allele Type:		Targeted (Conditional ready, No functional change)
Mutation:		Insertion
		Mutation details: A single loxP site was inserted 5' to the only coding exon, and a loxP-flanked hygromycin resistance cassette was inserted 3' to the exon. The hygromycin selection cassette was removed in ES cells by transient Cre expression leaving a single loxP site prior to the production of chimeric mice. (J:71969)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background
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hm1	Bdnf^tm3Jae/Bdnf^tm3Jae	involves: 129S4/SvJae
cn2	Bdnf^tm3Jae/Bdnf^tm3Jae	involves: 129S4/SvJae
cn3	Bdnf^tm3Jae/Bdnf^tm3Jae Tg(Cck-cre)CKres/0	involves: 129S4/SvJae * C57BL/6
cn4 Disease Model	Bdnf^tm3Jae/Bdnf^tm3Jae Mecp2^tm1.1Jae/Y Tg(Camk2a-cre)93Kln/0	involves: 129S4/SvJae * C57BL/6 * CBA/J
cn5	Bdnf^tm3Jae/Bdnf⁺ Mecp2^tm1.1Jae/Y Tg(Camk2a-cre)93Kln/0	involves: 129S4/SvJae * C57BL/6 * CBA/J
cn6	Bdnf^tm3Jae/Bdnf^tm3Jae Tg(Camk2a-cre)159Kln/0	involves: 129S4/SvJae * CBA/J
cn7	Bdnf^tm3Jae/Bdnf^tm3Jae Tg(Camk2a-cre)93Kln/0	involves: 129S4/SvJae * CBA/J
cn8	Bdnf^tm3Jae/Bdnf^tm3Jae Tg(Syn1-cre)671Jxm/0	involves: 129/Sv * C57BL/6 * ICR
cn9	Bdnf^tm3Jae/Bdnf^tm3Jae Tg(Eno2tTA)5030Nes/0 Tg(tetO-cre)1Jaw/0	involves: 129/Sv * C57BL/6 * ICR * SJL

Phenotypes:

Affected Systems	hm1	cn2	cn3	cn4	cn5	cn6	cn7		cn8	cn9
show or hide all annotated terms Sex:
behavior/neurological		√	√	√		√	√	√	√	√
behavior/neurological phenotype		N	N
lethargy				√
impaired behavioral response to xenobiotic										√
abnormal contextual conditioning behavior										√
abnormal cued conditioning behavior										√
impaired cued conditioning behavior		√	√
polyphagia							√	√
increased aggression towards male mice							√	√
increased anxiety-related response							√	√
limb grasping							√	√
hyperactivity						√	√	√		√
abnormal kindling response									√
growth/size/body						√	√	√
obese						√	√	√
increased body length							√	√
homeostasis/metabolism							√	√
abnormal homeostasis							√	√
increased circulating glucose level							√	√
increased circulating insulin level							√	√
increased circulating leptin level							√	√
insulin resistance							√	√
increased cholesterol level							√	√
mortality/aging				√	√
premature death				√	√
nervous system				√			√	√	√	√
abnormal kindling response									√
decreased brain weight							√	√
abnormal hippocampus CA2 region morphology							√	√
abnormal olfactory bulb morphology							√	√
abnormal action potential				√
reduced long-term potentiation										√
reproductive system							√
reduced female fertility							√
normal phenotype	√
no abnormal phenotype detected	√

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Key:

√

disease model

expected model not found

Models:

Human Diseases	cn4
Rett syndrome IDs Rett syndrome DOID:1206 ICD10CM:F84.2 MESH:D015518 NCI:C75488 OMIM:312750 OMIM:613454 UMLS_CUI:C0035372 Close	√

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 0 lines available
Carrying any Bdnf Mutation:	42 strains or lines available

References

Original:	J:71969 Rios M, et al., Conditional deletion of brain-derived neurotrophic factor in the postnatal brain leads to obesity and hyperactivity. Mol Endocrinol. 2001 Oct;15(10):1748-57
All:	112 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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