Eif2ak3<tm1Dron> Targeted Allele Detail MGI Mouse (MGI:2177666)

Eif2ak3^tm1Dron
Targeted Allele Detail

Summary

Symbol:	Eif2ak3^tm1Dron
Name:	eukaryotic translation initiation factor 2 alpha kinase 3; targeted mutation 1, David Ron
MGI ID:	MGI:2177666
Synonyms:	perk -
Gene:	Eif2ak3 Location: Chr6:70821499-70882229 bp, + strand Genetic Position: Chr6, 32.09 cM
Alliance:	Eif2ak3^tm1Dron page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:70005
Parent Cell Line:	W4 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutations:		Insertion, Intragenic deletion
		Mutation details: A neomycin selection cassette replaced 1.6 kb of DNA containing the transmembrane domain. Northern blot analysis demonstrated that at least 10-fold less transcript was produced from this allele that the wild-type. However, western blot analysis on cells derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:62257)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Expression

In Structures Affected by this Mutation:

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Eif2ak3 Mutation:	53 strains or lines available

References

Original:	J:62257 Harding HP, et al., Perk is essential for translational regulation and cell survival during the unfolded protein response. Mol Cell. 2000 May;5(5):897-904
All:	29 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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