Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:75954
Dunwoodie SL, et al., Axial skeletal defects caused by mutation in the spondylocostal dysplasia/pudgy gene Dll3 are associated with disruption of the segmentation clock within the presomitic mesoderm. Development. 2002 Apr;129(7):1795-806
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2178634
|
|
Gene symbol:
|
Sptbn1
|
|
Gene name:
|
spectrin beta, non-erythrocytic 1
|
|
Probe:
|
pSPORT-beta-spectrin2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
5G,H:
embryonic day 8.5; Dll3tm1Rbe/Dll3 +
(more  )
Specimen
5G,H:
(close  )
|
Genetic Background:
|
involves: 129P2/Ola * C57BL/6
|
|
Mutant Allele(s):
|
Dll3tm1Rbe/Dll3+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Bouin's Fixative
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: trunk somite |
Present |
Regionally restricted |
5H
,
5G
|
Authors state that expression in the center of somites marks epithelialization of mesenchyme to form somites.
|
Specimen
5I,K:
(close )
|
Genetic Background:
|
involves: 129P2/Ola * C57BL/6
|
|
Mutant Allele(s):
|
Dll3tm1Rbe/Dll3tm1Rbe
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Bouin's Fixative
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: trunk somite |
Present |
Regionally restricted |
5J
,
5I
|
Authors report that expression in somites is diffuse.
|
Specimen
5K,L:
(close )
|
Genetic Background:
|
involves: 129P2/Ola * C57BL/6
|
|
Mutant Allele(s):
|
Dll3tm1Rbe/Dll3tm1Rbe
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Bouin's Fixative
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: trunk somite |
Present |
Regionally restricted |
5K
,
5L
|
Authors report that expression in somites is diffuse.
|
|
Analysis Tools
