Hif1a<tm1Pec> Targeted Allele Detail MGI Mouse (MGI:2179429)

Hif1a^tm1Pec
Targeted Allele Detail

Summary

Symbol:	Hif1a^tm1Pec
Name:	hypoxia inducible factor 1, alpha subunit; targeted mutation 1, Peter Carmeliet
MGI ID:	MGI:2179429
Synonyms:	Hif1alpha^-, HIF-1alpha-
Gene:	Hif1a Location: Chr12:73948186-73994304 bp, + strand Genetic Position: Chr12, 31.99 cM
Alliance:	Hif1a^tm1Pec page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:76427
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutations:		Insertion, Intragenic deletion
		Mutation details: Exon 2 of this gene was replaced with a neomycin cassette via homologous recombination. Northern and Western blot analysis confirmed lack of gene expression in homozygous mutant embryonic stem (ES) cells. (J:76427)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Hif1a^tm1Pec

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Hif1a Mutation:	50 strains or lines available

Notes

Injection of homozygous ES cells into nude mice results in tumors that have reduced formation of large vessels and that have hypoxic microenvironments, however growth of tumors is not retarded but accelerated due to decreased hypoxia-induced apoptosis and increased stress-induced proliferation. Additionally, hypoxic and hypoglycemic conditions do not reduce proliferation and increase apoptosis in homozygous ES cells as in wild-type ES cells (J:76427).

References

Original:	J:76427 Carmeliet P, et al., Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature. 1998 Jul 30;394(6692):485-90
All:	14 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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