Slc23a2tm1Nbm
Targeted Allele Detail
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| Symbol: |
Slc23a2tm1Nbm |
| Name: |
solute carrier family 23 (nucleobase transporters), member 2; targeted mutation 1, Robert L Nussbaum |
| MGI ID: |
MGI:2180333 |
| Synonyms: |
Slc23a1-/-, SVCT2- |
| Gene: |
Slc23a2 Location: Chr2:131894416-131987028 bp, - strand Genetic Position: Chr2, 64.15 cM, cytoband G2
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| Alliance: |
Slc23a2tm1Nbm page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:76194
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| Parent Cell Line: |
TC1/TC-1 (ES Cell)
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| Strain of Origin: |
129S6/SvEvTac
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: 532 nucleotides of the gene spanning exons 10-12 were replaced with a neomycin resistance cassette via homologous recombination resulting in a deletion and frameshift. RT-PCR verified the presence of mutant transcript in brain of homozygous mutant animals. Disruption of protein function was verified via ascorbic-acid uptake assay. Mouse embryonic fibroblasts from homozygous mutant animals exhibit less than 5% of normal ascorbic-acid uptake compared to wild-type.
(J:76194)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:76194 Sotiriou S, et al., Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival. Nat Med. 2002 May;8(5):514-7 |
| All: |
14 reference(s) |
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Analysis Tools
