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Slc12a5tm1Tjj
Targeted Allele Detail
Summary
Symbol: Slc12a5tm1Tjj
Name: solute carrier family 12, member 5; targeted mutation 1, Thomas J Jentsch
MGI ID: MGI:2181025
Synonyms: Kcc2-
Gene: Slc12a5  Location: Chr2:164802766-164841651 bp, + strand  Genetic Position: Chr2, 85.28 cM, cytoband G2-G3
Alliance: Slc12a5tm1Tjj page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:69625
Parent Cell Line:  RW-4 (ES Cell)
Strain of Origin:  129X1/SvJ
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details: Exon 5 was replaced with a neomycin selection cassette, introducing a frameshift mutation. The deleted region encoded a portion of the second transmembrane domain. Northern analysis revealed a lack of transcript in homozygous mutant mice. Absence of a truncated protein product was confirmed by Western blot analysis using an antibody raised against the N-terminal. (J:69625)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 8 assay results
In Structures Affected by this Mutation: 3 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Slc12a5 Mutation:  45 strains or lines available
References
Original:  J:69625 Hubner CA, et al., Disruption of KCC2 Reveals an Essential Role of K-Cl Cotransport Already in Early Synaptic Inhibition. Neuron. 2001 May;30(2):515-24
All:  3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory