Dnmt3a<tm2Enl> Targeted Allele Detail MGI Mouse (MGI:2182412)

Dnmt3a^tm2Enl
Targeted Allele Detail

Summary

Symbol:	Dnmt3a^tm2Enl
Name:	DNA methyltransferase 3A; targeted mutation 2, En Li
MGI ID:	MGI:2182412
Gene:	Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
Alliance:	Dnmt3a^tm2Enl page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:58298
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutations:		Insertion, Intragenic deletion
		Mutation details: Targeting took place in ES cells heterozygous for Dnmt3a^tm1Enl, originally for the purpose of generating ES cells with both copies of the gene inactivated. A 0.3kb region of the wild-type allele encoding the PC and ENV motifs was replaced with a hygromycin selection cassette via homologous recombination. (J:58298)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:

4 assay results
1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Dnmt3a Mutation:	139 strains or lines available

References

Original:	J:58298 Okano M, et al., DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 1999 Oct 29;99(3):247-57
All:	11 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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