Slc22a1tm1Ahs
Targeted Allele Detail
|
|
| Symbol: |
Slc22a1tm1Ahs |
| Name: |
solute carrier family 22 (organic cation transporter), member 1; targeted mutation 1, Alfred H Schinkel |
| MGI ID: |
MGI:2182947 |
| Synonyms: |
Oct1- |
| Gene: |
Slc22a1 Location: Chr17:12867756-12894716 bp, - strand Genetic Position: Chr17, 8.63 cM
|
| Alliance: |
Slc22a1tm1Ahs page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:70604
|
| Parent Cell Line: |
E14 (ES Cell)
|
| Strain of Origin: |
129P2/OlaHsd
|
|
| Allele Type: |
|
Targeted (Null/knockout) |
| Mutations: |
|
Insertion, Intragenic deletion
|
| |
|
Mutation details: Exon 7 encoding putative transmembrane domains 7-9 and flanking intronic sequences were replaced with a PGK-hygro cassette in the reverse orientation via homologous recombination. Northern blot analysis did not detect gene expression in liver or kidney of homozygous mutant animals. However, RNase protection assay using a probe to nucleotides 447 to 691 lying outside the deleted region detected extremely low levels of transcript, which may represent a truncated and unstable message. Western blot analysis of homozygous mutant liver and kidney samples using a polyclonal antibody probe recognizing the C-terminal region of rat and mouse protein further verified the absence of gene expression.
(J:70604)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Slc22a1 Mutation: |
31 strains or lines available
|
|
| Original: |
J:70604 Jonker JW, et al., Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene. Mol Cell Biol. 2001 Aug;21(16):5471-7 |
| All: |
8 reference(s) |
|
Analysis Tools
