Mbd4tm1Bird
Targeted Allele Detail
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| Symbol: |
Mbd4tm1Bird |
| Name: |
methyl-CpG binding domain protein 4; targeted mutation 1, Adrian Bird |
| MGI ID: |
MGI:2183491 |
| Synonyms: |
Mbd4- |
| Gene: |
Mbd4 Location: Chr6:115817658-115830332 bp, - strand Genetic Position: Chr6, 53.72 cM
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| Alliance: |
Mbd4tm1Bird page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:77896
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| Parent Cell Line: |
E14TG2a (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutation: |
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Insertion
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Mutation details: A cassette, containing a splice acceptor site and IRES-Beta-geo, was inserted into intron 1 of the endogenous locus. Northern blot and RT-PCR analysis showed levels of transcript in homozygous mutant mice to be less than 0.1% of those in wild-type mice. Two truncated transcripts were identified; one which truncated at the transgenic polyadenylation signal and another in which a frameshift mutation was introduced by the excision of the IRES-Beta-geo transgenic region (leaving the the splice acceptor site in between exons 1 and 2).
(J:77896)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Mbd4 Mutation: |
32 strains or lines available
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| Original: |
J:77896 Millar CB, et al., Enhanced CpG mutability and tumorigenesis in MBD4-deficient mice. Science. 2002 Jul 19;297(5580):403-5 |
| All: |
3 reference(s) |
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Analysis Tools
