Gene Expression Data
Assay Details
Assay
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Reference:
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J:25250
Grindley JC, et al., The role of Pax-6 in eye and nasal development. Development. 1995 May;121(5):1433-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2385724
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3A:
embryonic day 8.0
(more )
Specimen
3A:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 8.0
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Specimen
3B:
embryonic day 8.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Swiss
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Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future prosencephalon |
Present |
Regionally restricted |
3B
|
Segmented expression in presumptive diencephalon, mainly dorsal, reaching neural folds segmented. Weaker in presumptive telencephalon; neural ectoderm expression weak or undetectable at neural folds.
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TS13: future prosencephalon neural fold |
Present |
Regionally restricted |
3B
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Segmented expression in presumptive diencephalon, mainly dorsal, reaching neural folds segmented. Weaker in presumptive telencephalon; neural ectoderm expression weak or undetectable at neural folds.
|
TS13: optic pit neural ectoderm |
Strong |
Regionally restricted |
3B
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Authors report that expression is strong in optic vesicle,
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TS13: surface ectoderm |
Present |
Regionally restricted |
3B
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Expression in surface ectoderm is still broad, and extends rostrally to neural folds.
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Specimen
3C:
embryonic day 9.25
(more )
Specimen
3C:
(close )
Genetic Background:
|
Swiss
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Age:
|
embryonic day 9.25
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: lens placode |
Present |
Regionally restricted |
3C
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Expression in lens placode forms part of a larger domain that extends in dorsal-caudal direction.
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Specimen
3D:
embryonic day 9.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Swiss
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Age:
|
embryonic day 9.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: lens pit |
Present |
Regionally restricted |
3D
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Expression in surface ectoderm becomes restricted to developing lens placode, nasal placode and immediately adjacent tissue.
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TS16: nasal pit |
Present |
Regionally restricted |
3D
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Expression in surface ectoderm becomes restricted to developing lens placode, nasal placode and immediately adjacent tissue.
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TS16: surface ectoderm |
Present |
Regionally restricted |
3D
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Expression in surface ectoderm becomes restricted to developing lens placode, nasal placode and immediately adjacent tissue.
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Specimen
not shown:
embryonic day 9.75
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 9.75
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: lens pit |
Present |
Regionally restricted |
|
Authors report expression in lens and nasal placodes, clearly separated by a region of non-expressing ectoderm.
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TS16: nasal pit |
Present |
Regionally restricted |
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Authors report expression in lens and nasal placodes, clearly separated by a region of non-expressing ectoderm.
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TS16: surface ectoderm |
Present |
Regionally restricted |
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Authors report expression in lens and nasal placodes, clearly separated by a region of non-expressing ectoderm.
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Specimen
3E:
embryonic day 10.5
(more )
Specimen
3E:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: eye surface ectoderm |
Present |
Regionally restricted |
3E
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As lens pit formation proceeds, expression domain is further restricted until it lies exclusively within developing eye region.
|
TS17: lens pit |
Present |
Regionally restricted |
3E
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As lens pit formation proceeds, expression domain is further restricted until it lies exclusively within developing eye region.
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Specimen
3F:
embryonic day 15.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cornea |
Present |
Regionally restricted |
3F
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Expression continues in parts of eye derived from surface ectoderm, including lens pit, lens vesicle and lens, as well as developing cornea.
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TS23: eye |
Present |
Regionally restricted |
3F
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Expression continues in parts of eye derived from surface ectoderm, including lens pit, lens vesicle and lens, as well as developing cornea.
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TS23: lens |
Present |
Regionally restricted |
3F
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Expression continues in parts of eye derived from surface ectoderm, including lens pit, lens vesicle and lens, as well as developing cornea.
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Specimen
4C:
(close )
Genetic Background:
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involves: Swiss
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
|
embryonic day 15.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: eye |
Present |
Regionally restricted |
4C
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Authors report expression predominantly in 2 neuroectodermal layers of optic cup-like structure.
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TS23: optic stalk |
Weak |
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4C
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Little expression in optic stalk.
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Specimen
4D:
embryonic day 12.5
(more )
Specimen
4D:
(close )
Genetic Background:
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Swiss
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Specimen
4E:
(close )
Genetic Background:
|
involves: Swiss
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Mutant Allele(s):
|
Pax6Sey/Pax6Sey
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: eye |
Present |
Regionally restricted |
4E
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Authors describe distally restricted expression domains.
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Specimen
not shown:
(close )
Genetic Background:
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involves: Swiss
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
|
embryonic day 8.0
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: eye |
Present |
Regionally restricted |
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Authors report no differences from normal pattern of expression in mutant embryo eye.
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Specimen
5A:
embryonic day 8.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: eye |
Present |
|
5A
|
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Specimen
5B:
(close )
Genetic Background:
|
involves: Swiss
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Mutant Allele(s):
|
Pax6Sey/Pax6Sey
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Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: eye |
Present |
Regionally restricted |
5B
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Authors report that homozygous mutant embryos have the same expression pattern in optic vesicles as their +/+ and +/- littermates.
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TS13: optic pit |
Present |
Regionally restricted |
5B
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Authors report that homozygous mutant embryos have the same expression pattern in optic vesicles as their +/+ and +/- littermates.
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Specimen
5C control:
embryonic day 9.75
(more )
Specimen
5C control:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 9.75
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: surface ectoderm |
Present |
Regionally restricted |
5C (right)
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Expression in surface ectoderm adjacent to optic vesicle; strongest in most distal structures; confined to around lens and nasal placodes.
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Specimen
5C Sey/Sey:
(close )
Genetic Background:
|
involves: Swiss
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Mutant Allele(s):
|
Pax6Sey/Pax6Sey
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Age:
|
embryonic day 9.75
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: surface ectoderm |
Absent |
|
5C (left)
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No expression in surface ectoderm anywhere in the head, including the eye region; no expression in surface ectoderm adjacent to optic vesicle.
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Specimen
6A:
embryonic day 9.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: lens placode |
Present |
|
6A
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Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
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TS15: nose |
Present |
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6A
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Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
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TS15: olfactory placode |
Present |
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6A
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Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
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Specimen
6B:
embryonic day 10.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: nasal pit |
Present |
Regionally restricted |
6B
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Expression in nasal region; continues in placodal epithelium during formation of nasal pits.
|
TS17: nose |
Present |
Regionally restricted |
6B
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Expression in nasal region; continues in placodal epithelium during formation of nasal pits.
|
Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6C:
embryonic day 9.75
(more )
Specimen
6C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 9.75
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: nose |
Present |
Regionally restricted |
6C
|
Authors report expression detectable in nasal placodes.
|
Specimen
6D:
(close )
Genetic Background:
|
involves: Swiss
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 9.75
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: nose |
Absent |
|
6D
|
Authors report absence of expression in nasal placode and nasal region.
|
TS16: future forebrain |
Present |
|
6D
|
Expression in forebrain neuroepithelium.
|
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