Myh10tm1Rsad
Targeted Allele Detail
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| Symbol: |
Myh10tm1Rsad |
| Name: |
myosin, heavy polypeptide 10, non-muscle; targeted mutation 1, Robert S Adelstein |
| MGI ID: |
MGI:2386439 |
| Synonyms: |
BdeltaI |
| Gene: |
Myh10 Location: Chr11:68582385-68707458 bp, + strand Genetic Position: Chr11, 41.95 cM, cytoband B3
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| Alliance: |
Myh10tm1Rsad page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:60967
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| Parent Cell Line: |
J1 (ES Cell)
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| Strain of Origin: |
129S4/SvJae
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| Allele Type: |
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Targeted (Hypomorph, Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A 30 bp neural-specific exon referred to as N30 (also called the B1 insert) and the flanking introns were replaced with a PGK-neo cassette via homologous recombination. Deletion of the B1 insert was confirmed by RNA analysis of neural tissues. Northern blot of various tissues from homozygous mutant mice showed a significant decrease in gene expression. Western blot analysis showed a significant decrease in protein expression in both embryonic and adult mutant mice, including a decrease of 88% in the heart, of 65% in the brain, and of 64% in the lung. This is a mildly hypomorphic allele.
(J:60967)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:60967 Uren D, et al., Gene dosage affects the cardiac and brain phenotype in nonmuscle myosin II-B-depleted mice. J Clin Invest. 2000 Mar;105(5):663-71 |
| All: |
2 reference(s) |
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Analysis Tools
