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Myh10tm2Rsad
Targeted Allele Detail
Summary
Symbol: Myh10tm2Rsad
Name: myosin, heavy polypeptide 10, non-muscle; targeted mutation 1, Robert S Adelstein
MGI ID: MGI:2386445
Synonyms: B-, NMHC-B-
Gene: Myh10  Location: Chr11:68582385-68707458 bp, + strand  Genetic Position: Chr11, 41.95 cM, cytoband B3
Alliance: Myh10tm2Rsad page
Cardiac defects in newborn Myh10tm2Rsad/Myh10tm2Rsad mice

Show the 3 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:44181
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation details: The gene was disrupted by insertion of a PGK-neo cassette into exon 2 just downstream of the translation initiation codon via homologous recombination. Northern blot analysis of brain RNA from mutant animals detected a 0.9 kb mutant transcript containing the PGK-neo insertion. Western blot analysis of several tissues from newborn homozygous mutant animals showed an absence of protein product. (J:44181)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 40 assay results
In Structures Affected by this Mutation: 11 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myh10 Mutation:  95 strains or lines available
References
Original:  J:44181 Tullio AN, et al., Nonmuscle myosin II-B is required for normal development of the mouse heart. Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12407-12
All:  18 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory