Antibody:	anti-Nr-CAM
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS19: neural tube marginal layer	Present	Regionally restricted	3A	Staining is present in the commissural cell axons. Staining is especially strong in the ventral commissure where is crosses the floor plate.
TS19: neural tube floor plate	Present		3A
TS19: neural tube mantle layer	Weak	Regionally restricted	3A	There is weak staining in the radial glia.

Structure	Level	Pattern	Image	Note
TS19: neural tube marginal layer	Present	Regionally restricted	3D	Staining is present in the ventral commissure, especially where it crosses the floor plate.
TS19: neural tube floor plate	Present		3D
TS19: neural tube mantle layer	Weak	Regionally restricted	3D	There is weak staining in the radial glia.

Structure	Level	Pattern	Image	Note
TS20: spinal cord marginal layer	Present	Regionally restricted	3G	Staining is present in the commissural cell axons. Staining is especially strong in the ventral commissure where is crosses the floor plate. There is no staining in the dorsal root entry zone.
TS20: spinal cord floor plate	Present		3G

Structure	Level	Pattern	Image	Note
TS22: spinal cord marginal layer	Present	Regionally restricted	3J	There is strong staining in the ventral commissure. There is weak staining in the ventrolateral region of the dorsal root entry zone and in the longitudinal fiber tracts. There is no staining in the precommissural C cell fibers.
TS22: spinal cord floor plate	Present		3J

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted		Staining is present in the habenula and habenular commissure but not in the posterior commissure.
TS24: cerebral cortex	Present	Regionally restricted		Staining is present in the anterior commissure in the part that contains fibers from the olfactory bulb.
TS24: spinal cord floor plate	Present
TS24: spinal cord marginal layer	Present	Regionally restricted		Staining is present in the ventral commissure and in the dorsal root entry zone.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Strong	Regionally restricted	6A	Staining is present at the ventral midline.

Structure	Level	Pattern	Image	Note
TS24: median eminence	Present		6B
TS24: diencephalon	Present	Regionally restricted	6B	Staining is present in the midline cells at the floor of the ventricle.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	6D	In the region above the pituitary, staining is present in radial fibers extending from the third ventricle to the pia.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted		Staining is present in the ventral hindbrain at the level of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Present	Regionally restricted		Staining is present at the midline, as well as in the habenula and its commissure.
TS27: median eminence	Present

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	7A	Staining is present in the commissure of the superior colliculus and in the posterior commissure.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	7B	Staining is present in the mammillary body.
TS24: midbrain	Present	Regionally restricted	7B	Staining is present in the commissure of the superior colliculus and the posterior commissure.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	7C	There is no staining in the corpus callosum.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	7F	Staining is present in the corpus callosum.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	8A	Staining is present in the habenulointerpeduncular tract.
TS22: midbrain	Present	Regionally restricted	8A	Staining is present in the commissure of the superior colliculus.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted		Staining is present in the habenula and the habenula commissure.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	8C	Staining is present in the habenulointerpeduncular tract.
TS24: midbrain	Present	Regionally restricted	8C	Staining is present in the posterior commissure.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	8E	Staining is present in the anterior portion of the anterior commissure (in the region that contains fibers from the olfactory bulb) but not in the posterior portion.
TS27: telencephalon	Present	Regionally restricted	8E	Staining is present in the lateral olfactory tracts.
TS27: optic nerve	Present		8E

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Present	Regionally restricted		Staining is present in the habenula, its commissure, and the habenulointerpeduncular tract.
TS27: telencephalon	Present	Regionally restricted		Staining is present in the lateral olfactory tract.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present			Expression is present in the striamedullaris/thalamic region.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	9A	Staining is present in the external and internal plexiform layers and in the granule cell layer.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present	Regionally restricted	9C	Staining is present in the pyramidal cell layer, granule cell layer in the dentate gyrus, and in the molecular layer. It is not present in the alveus.