Adra1atm1Pcs
Targeted Allele Detail
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| Symbol: |
Adra1atm1Pcs |
| Name: |
adrenergic receptor, alpha 1a; targeted mutation 1, Paul C Simpson |
| MGI ID: |
MGI:2387663 |
| Gene: |
Adra1a Location: Chr14:66872700-67008617 bp, + strand Genetic Position: Chr14, 34.6 cM, cytoband D1
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| Alliance: |
Adra1atm1Pcs page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:77732
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| Parent Cell Line: |
RW-4 (ES Cell)
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| Strain of Origin: |
129X1/SvJ
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Exon 1 and 415 bp of intron 1 were replaced with a cassette containing lacZ and neo genes. The deleted region encoded the receptor and transmembrane regions 1 through 5. The absence of transcript was confirmed by both RT-PCR analysis and RNAse protection. Binding assays showed adrenergic receptor binding to be reduced by approximately 50% in brain and kidney tissues and by 70% in heart tissue, all obtained from homozygous mutant mice. Expression of beta-galactosidase was driven by the endogenous promoter.
(J:77732)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:77732 Rokosh DG, et al., Knockout of the alpha 1A/C-adrenergic receptor subtype: the alpha 1A/C is expressed in resistance arteries and is required to maintain arterial blood pressure. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9474-9 |
| All: |
21 reference(s) |
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Analysis Tools
