Smarca2tm1Mya
Targeted Allele Detail
|
|
| Symbol: |
Smarca2tm1Mya |
| Name: |
SWI/SNF related BAF chromatin remodeling complex subunit ATPase 2; targeted mutation 1, Moshe Yaniv |
| MGI ID: |
MGI:2387988 |
| Synonyms: |
BRM- |
| Gene: |
Smarca2 Location: Chr19:26582578-26755721 bp, + strand Genetic Position: Chr19, 21.17 cM, cytoband C1
|
| Alliance: |
Smarca2tm1Mya page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:51536
|
| Parent Cell Line: |
CK35 (ES Cell)
|
| Strain of Origin: |
129S2/SvPas
|
|
| Allele Type: |
|
Targeted (Null/knockout) |
| Mutations: |
|
Insertion, Intragenic deletion
|
| |
|
Mutation details: The majority of the exon encoding amino acids 120-176 was replaced with a neomycin resistance cassette via homologous recombination. RT-PCR analysis of liver RNA from homozygous mutant animals revealed a faint truncated transcript resulting from an alternative splice product which skips the disrupted exon. Western blot analysis of brain nuclear extracts detected a shortened protein product expressed at less than 1% the amount of wild-type protein. Co-immunoprecipitation experiments showed that the mutant protein lacked functional capabilities.
(J:51536)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Smarca2 Mutation: |
90 strains or lines available
|
|
| Original: |
J:51536 Reyes JC, et al., Altered control of cellular proliferation in the absence of mammalian brahma (SNF2alpha). EMBO J. 1998 Dec 1;17(23):6979-91 |
| All: |
18 reference(s) |
|
Analysis Tools
