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Raf1tm1Bacc
Targeted Allele Detail
Summary
Symbol: Raf1tm1Bacc
Name: v-raf-leukemia viral oncogene 1; targeted mutation 1, Manuela Baccarini
MGI ID: MGI:2388026
Synonyms: c-raf-1-, Raf-1-
Gene: Raf1  Location: Chr6:115595530-115653596 bp, - strand  Genetic Position: Chr6, 53.62 cM, cytoband C3
Alliance: Raf1tm1Bacc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:69056
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation details: Exon 3 was flanked with loxP sites by insertion of a floxed TK-neo cassette and a single loxP site into the upstream and downstream introns, respectively. Exon 3 was removed by transient expression of cre recombinase in correctly targeted ES cells. Absence of gene expression was confirmed by Western blot analysis of mouse embryonic fibroblasts (MEFs) derived from homozygous mutant embryos. Immune complex kinase assays showed a lack of enzyme activity in MEFs from homozygous mutants. (J:69056)
Phenotypes
Key:
hm homozygous ht heterozygous tg involves transgenes √ phenotype observed
cn conditional genotype  cx complex: > 1 genome feature ot other: hemizygous, indeterminate,... N normal phenotype
Genotype/
Background:
Allelic Composition
Genetic Background
Cell Line(s)
Allelic CompositionGenetic BackgroundCell Line(s)
hm1
Raf1tm1Bacc/Raf1tm1Bacc
involves: 129P2/OlaHsd * 129/Sv
 
hm2
Raf1tm1Bacc/Raf1tm1Bacc
involves: 129P2/OlaHsd * C57BL/6
 
cn3
Braftm1Sva/Braftm1.1Sva
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
 
cn4
Braftm1Wds/Braftm1.1Wds
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
 
cx5
Faslpr/Fas+
Raf1tm1Bacc/Raf1tm1Bacc
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL
 
cx6
Faslpr/Faslpr
Raf1tm1Bacc/Raf1tm1Bacc
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL
 
cx7
Faslpr/Fas+
Raf1tm1Bacc/Raf1+
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL
 
Phenotypes:
Affected Systems
hm1
hm2
cn3
cn4
cx5
cx6
cx7
show or hide all annotated terms
             
cardiovascular system
√ √
abnormal liver sinusoid morphology
√ √
abnormal placental labyrinth vasculature morphology
√ √
cellular
√ √ √ √ √
decreased sensitivity to induced cell death
√ √
increased sensitivity to induced cell death
√ √
increased apoptosis
√ √
increased fibroblast apoptosis
√ √
increased hepatoblast apoptosis
√ √
abnormal axon extension
√ √
embryo
√ √
embryonic growth retardation
√ √
decreased embryo size
√ √
decreased embryo weight
√ √
decreased spongiotrophoblast size
√ √
abnormal placenta labyrinth morphology
√ √
abnormal placental labyrinth vasculature morphology
√ √
small placenta
√ √
decreased placenta weight
√ √
growth/size/body
√ √
embryonic growth retardation
√ √
decreased embryo size
√ √
decreased embryo weight
√ √
hematopoietic system
√ √ N
hematopoietic system phenotype
N N
abnormal erythropoiesis
√ √
integument
√ √
pallor
√ √
liver/biliary system
√ √
abnormal liver sinusoid morphology
√ √
increased hepatoblast apoptosis
√ √
abnormal hepatocyte morphology
√ √
small liver
√ √
liver hypoplasia
√ √
pale liver
√ √
mortality/aging
√ √ √ √ N
mortality/aging
N
lethality throughout fetal growth and development, complete penetrance
√
embryonic lethality during organogenesis, complete penetrance
√ √ √
nervous system
√ √
abnormal axon extension
√ √
abnormal innervation
√ √
abnormal dorsal root ganglion morphology
√ √
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Raf1 Mutation:  117 strains or lines available
References
Original:  J:69056 Mikula M, et al., Embryonic lethality and fetal liver apoptosis in mice lacking the c-raf-1 gene. EMBO J. 2001 Apr 17;20(8):1952-62
All:  6 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory