Slc22a3tm1Dpb
Targeted Allele Detail
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| Symbol: |
Slc22a3tm1Dpb |
| Name: |
solute carrier family 22 (organic cation transporter), member 3; targeted mutation 1, Denise P Barlow |
| MGI ID: |
MGI:2388117 |
| Synonyms: |
Oct3-, Orct3-deficient |
| Gene: |
Slc22a3 Location: Chr17:12638859-12726591 bp, - strand Genetic Position: Chr17, 8.52 cM
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| Alliance: |
Slc22a3tm1Dpb page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:69978
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| Parent Cell Line: |
E14 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Exon 1 and flanking intronic sequences were deleted and replaced with a floxed PGK-neo cassette via homologous recombination. Transient expression of cre recombinase in correctly targeted cells removed the selection cassette leaving behind a single loxP site in place of exon 1. Gene expression was absent in the heart, brain, and skeletal muscle of homozygous mutant animals as determined by Northern blot analysis. However, an aberrant transcript was detected in placentas from homozygous mutant embryos. RT-PCR analysis revealed the mutant transcript results from alternative splicing of intron 2 to exon 3 and does not translate a functional protein product.
(J:69978)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Slc22a3 Mutation: |
29 strains or lines available
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| Original: |
J:69978 Zwart R, et al., Impaired activity of the extraneuronal monoamine transporter system known as uptake-2 in Orct3/Slc22a3-deficient mice. Mol Cell Biol. 2001 Jul;21(13):4188-96 |
| All: |
13 reference(s) |
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Analysis Tools
