Psme1/Psme2tm1Tchi
Targeted Allele Detail
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| Symbol: |
Psme1/Psme2tm1Tchi |
| Name: |
proteasome (prosome, macropain) activator subunit 2 (PA28 beta); targeted mutation 1, Tomoki Chiba |
| MGI ID: |
MGI:2388601 |
| Synonyms: |
PA28alpha-beta- |
| Gene: |
Psme2 Location: Chr14:55824897-55828558 bp, - strand Genetic Position: Chr14, 28.19 cM, cytoband C2-D1
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| Alliance: |
Psme1/Psme2tm1Tchi page
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A targeting construct where exons 2-10 of Psme1 were replaced with a neomycin resistant cassette and exons 2-10 of Psme2 were deleted was used to disrupt both loci in a single homologous recombination event. The sequence of Cgi112 (1500005A01Rik), the gene located between Psme1 and Psme2, remained unchanged. Northern blot and Western blot analysis of mouse embryonic fibroblast (MEFs) cultured from homozygous mutant embryos confirmed the absence of Psme1 and Psme2 gene expression.
(J:72533)
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View phenotypes and curated references for all genotypes (concatenated display).
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Homozygous mutant animals breed well and appear healthy with no observable abnormalities.
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| Original: |
J:72533 Murata S, et al., Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta. EMBO J. 2001 Nov 1;20(21):5898-907 |
| All: |
12 reference(s) |
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Analysis Tools
