Gclctm1Dwn
Targeted Allele Detail
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| Symbol: |
Gclctm1Dwn |
| Name: |
glutamate-cysteine ligase, catalytic subunit; targeted mutation 1, Daniel W Nebert |
| MGI ID: |
MGI:2429419 |
| Synonyms: |
Gclc(-) |
| Gene: |
Gclc Location: Chr9:77661817-77701767 bp, + strand Genetic Position: Chr9, 43.36 cM, cytoband D-E
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| Alliance: |
Gclctm1Dwn page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:66499
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| Parent Cell Line: |
E14TG2a (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: An Hprt mini gene replaced a portion of exon 4 and all of exons 5 and 6. While translation of the targeted allele may be possible, in which case a truncated protein lacking all but 161 of the 637 amino acids would be produced, Western blot analysis showed protein levels to be decreased by approximately 50% in heterozygous mice. An assay of endogenous enzyme activity correspondingly showed an approximate 50% reduction in hepatic tissue obtained from heterozygotes.
(J:66499)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Gclc Mutation: |
20 strains or lines available
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| Original: |
J:66499 Dalton TP, et al., Knockout of the mouse glutamate cysteine ligase catalytic subunit (Gclc) gene: embryonic lethal when homozygous, and proposed model for moderate glutathione deficiency when heterozygous. Biochem Biophys Res Commun. 2000 Dec 20;279(2):324-9 |
| All: |
1 reference(s) |
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Analysis Tools
