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Gja1tm1Kwi
Targeted Allele Detail
Summary
Symbol: Gja1tm1Kwi
Name: gap junction protein, alpha 1; targeted mutation 1, Klaus Willecke
MGI ID: MGI:2445468
Synonyms: 3lox, Cx43fl, Cx43fl[lacZ], Cx43::LacZ
Gene: Gja1  Location: Chr10:56253297-56266519 bp, + strand  Genetic Position: Chr10, 28.64 cM
Alliance: Gja1tm1Kwi page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:78662
Parent Cell Line:  HM-1 (ES Cell)
Strain of Origin:  129P2/OlaHsd-Hprt1b-m3
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation details: LoxP sites flanking the coding region of the gene were introduced into exon 2 and a floxed tk-neo cassette and lacZ gene were inserted into the downstream region via homologous recombination. Northern blot analysis confirmed expression of the mutant allele, and Western blot analysis showed protein expression is similar in mutant animals compared to wild-type. (J:78662)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 7 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gja1 Mutation:  60 strains or lines available
References
Original:  J:78662 Theis M, et al., Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene. Genesis. 2001 Jan;29(1):1-13
All:  114 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory