Gene Expression Data
Assay Details
Assay
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Reference:
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J:36833
Joseph SJ, et al., A proteoglycan that activates fibroblast growth factors during early neuronal development is a perlecan variant. Development. 1996 Nov;122(11):3443-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2447889
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Gene symbol:
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Hspg2
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Gene name:
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perlecan (heparan sulfate proteoglycan 2)
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Antibody:
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Anti-HSPG-PRM
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
7A:
embryonic day 16.5
(more )
Note: The plane of section was horizontal.
Specimen
7A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
|
Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was horizontal.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diencephalon ventricular layer |
Present |
Regionally restricted |
7A
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Expression was detected in the neural precursor cells lining the inner face of the ventricular zone.
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TS24: brain |
Present |
Regionally restricted |
7A
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Expression was detected in the endothelial cells of blood vessels in the neuroepithelium.
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Specimen
7C:
embryonic day 16.5
(more )
Note: The plane of section was horizontal.
Specimen
7C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
The plane of section was horizontal.
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Specimen
8A:
embryonic day 9.0
(more )
Note: The plane of section was horizontal from high cervical levels.
Specimen
8A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 9.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
The plane of section was horizontal from high cervical levels.
|
Specimen
8B:
embryonic day 10.0
(more )
Note: The plane of section was horizontal from high cervical levels.
Specimen
8B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
The plane of section was horizontal from high cervical levels.
|
Specimen
8D:
embryonic day 12.0
(more )
Note: The plane of section was horizontal.
Specimen
8D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
The plane of section was horizontal.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube |
Present |
Regionally restricted |
8D
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Expression was detected in the neuroepithelial cells lining the lumen.
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TS19: future spinal cord |
Present |
Regionally restricted |
8D
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Expression was detected in the dividing lateral wall/ventricular layer cells lining the central canal and the basement membrane.
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TS19: embryo mesenchyme |
Present |
Regionally restricted |
8D
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Expression was detected surrounding the spinal cord.
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TS19: cardiovascular system |
Present |
Regionally restricted |
8D
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Expression was detected in the endothelial cells of blood vessels of the neuroepithelium.
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Specimen
8E:
embryonic day 16.0
(more )
Note: The plane of section was horizontal from high cervical levels.
Specimen
8E:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 16.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
The plane of section was horizontal from high cervical levels.
|
Specimen
9:
embryonic day 16.0
(more )
Note: The plane of section was horizontal.
Specimen
9:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
The plane of section was horizontal.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: olfactory lobe |
Present |
Regionally restricted |
9
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Expression was detected in the processes of the olfactory epithelium, the basement membrane and was strongly detected in the extracellular matrix.
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Specimen
10:
embryonic day 10.0
(more )
Note: The plane of section was sagittal.
Specimen
10:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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The plane of section was sagittal.
|
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