About   Help   FAQ
Car9tm1Spas
Targeted Allele Detail
Summary
Symbol: Car9tm1Spas
Name: carbonic anhydrase 9; targeted mutation 1, Silvia Pastorekova
MGI ID: MGI:2448129
Synonyms: CA IX KO, Car9-
Gene: Car9  Location: Chr4:43507026-43513729 bp, + strand  Genetic Position: Chr4, 23.05 cM
Alliance: Car9tm1Spas page
Morphological changes in the stomach and intestine of Car2n/Car2n and Car9tm1Spas/Car9tm1Spas mice

Show the 1 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:80329
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details: A PGK-neo cassette was inserted into exon 1 by homologous recombination. The insertion of neo downstream of the signal peptide deleted 14 nt (268-282) of the proteoglycan domain and caused a frameshift mutation. RT-PCR analysis of homozygous mutant mice identified a frame shifted transcript lacking 5' sequence encoding the signal peptide and most of the proteoglycan domain. Though the mutant transcript contained 77 nt of the neo cassette, a 3' portion of exon 1, and all of exons 2 through 11, protein was undetected by Western blot analysis of stomach tissue. (J:80329)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Car9 Mutation:  46 strains or lines available
References
Original:  J:80329 Gut MO, et al., Gastric hyperplasia in mice with targeted disruption of the carbonic anhydrase gene Car9. Gastroenterology. 2002 Dec;123(6):1889-903
All:  10 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory