Tg(APP695)3Dbo Transgene Detail MGI Mouse (MGI:2447334)

Tg(APP695)3Dbo
Transgene Detail

Summary

Symbol:	Tg(APP695)3Dbo
Name:	transgene insertion 3, David R Borchelt
MGI ID:	MGI:2447334
Synonyms:	APP695, APP695swe, APPswe, line C3-3, Mo/HuAPPswe
Transgene:	Tg(APP695)3Dbo Location: unknown
Alliance:	Tg(APP695)3Dbo page

Transgene
origin

Strain of Origin:

(C57BL/6J x C3H/HeJ)F2

Transgene
description

Transgene Type:

Transgenic (Humanized sequence, Inserted expressed sequence)

Mutation:

Insertion

Tg(APP695)3Dbo expresses 1 gene

Mutation details: The transgene is composed of a cDNA encoding a chimeric APP protein regulated by the mouse prion promoter. The chimeric APP molecule was created by replacing sequences encoding the Abeta domain of a 695 amino acid isoform of the murine sequence with the cognate sequences of the human gene (mutations K595N, M596L). The human mutations are found in familial Alzheimer's disease. Transgene expression was observed in the brain and heart by Western blot analysis using a monoclonal antibody recognizing the human Abeta region. (J:80782)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available Cell Lines: 0 lines available

Notes

Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 (Tg(Prnp-App/APPswe)E1-2Dbo) and C3-3.

This line was generated from founder number C3-3.

Transgenic mice develop amyloid deposits in brain tissue by 18-20 months of age.

Transgenic mice that are also transgenic for Tg(PSEN1)5Dbo express both human presenilin 1 (A246E variant) and a chimeric amyloid precursor protein (APPSwe) under direction of the mouse prion protein promoter. Elevated levels of the AB1-42(43) peptide are detected in brain homogenates. By nine months of age, histological examination of brain tissue from these mice reveals numerous amyloid deposits resembling those observed in the brains of patients with Alzheimer's disease (AD). The number of amyloid deposits increases dramatically between the ages of 10 and 12 months.

References

Original:	J:80782 Borchelt DR, et al., A vector for expressing foreign genes in the brains and hearts of transgenic mice. Genet Anal. 1996 Dec;13(6):159-63
All:	149 reference(s)

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