Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:51129
Bierkamp C, et al., Desmosomal localization of beta-catenin in the skin of plakoglobin null-mutant mice. Development. 1999 Jan;126(2):371-81
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:2449090
|
Gene symbol:
|
Dsc2
|
Gene name:
|
desmocollin 2
|
Antibody:
|
anti-Desmocollins
|
Detection system:
|
Secondary antibody coupled to colloidal gold
|
Assay notes:
|
The tissue was fixed in 3% paraformaldehyde for 10 min., followed by 8% paraformaldehyde overnight.
|
|
Results
|
Specimen
5G:
embryonic day 17.5
(more )
Note: Embedded in methacrylate.
Specimen
5G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embedded in methacrylate.
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: gut |
Present |
Regionally restricted |
5G
|
Expression was detected in the desmosomes of intestinal epithelium.
|
Specimen
5H:
embryonic day 17.5; Juptm1Kem/ Juptm1Kem
(more )
Note: Embedded in methacrylate.
Specimen
5H:
(close )
Genetic Background:
|
either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
|
Mutant Allele(s):
|
Juptm1Kem/Juptm1Kem
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embedded in methacrylate.
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: gut |
Present |
Regionally restricted |
5H
|
Expression was detected in the desmosomes and in the membrane outside of desmosomes of intestinal epithelium.
|
|