Chat<tm1Fhg> Targeted Allele Detail MGI Mouse (MGI:2450310)

Chat^tm1Fhg
Targeted Allele Detail

Summary

Symbol:	Chat^tm1Fhg
Name:	choline O-acetyltransferase; targeted mutation 1, Fred H Gage
MGI ID:	MGI:2450310
Synonyms:	ChAT(-)
Gene:	Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
Alliance:	Chat^tm1Fhg page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:81738
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Mutation
description

Allele Type:		Targeted (Null/knockout)
Mutations:		Insertion, Intragenic deletion
		Mutation details: Exons 11, 12, and 13 were replaced with a neomycin selection cassette inserted by homologous recombination. Normal protein was undetected by immunostaining of the basal forebrain of homozygous mutant embryos. Assays of the brain stem, spinal cord, and septum showed an absence of endogenous activity. (J:81738)
Inheritance:		Recessive

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Expression

In Structures Affected by this Mutation:

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Chat Mutation:	58 strains or lines available

References

Original:	J:81738 Brandon EP, et al., Aberrant patterning of neuromuscular synapses in choline acetyltransferase-deficient mice. J Neurosci. 2003 Jan 15;23(2):539-49
All:	11 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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