Gene Expression Data
Assay Details
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Assay
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Reference:
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J:76911
Xu J, et al., Sex differences in sex chromosome gene expression in mouse brain. Hum Mol Genet. 2002 Jun 1;11(12):1409-19
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Assay type:
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Northern blot
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MGI Accession ID:
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MGI:2651524
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Gene symbol:
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Kdm5d
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Gene name:
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lysine demethylase 5D
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Probe:
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Smcx/y
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Probe preparation:
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Antisense labelled with P32 DNA
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Visualized with:
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Phosphorimaging
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Assay notes:
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This probe is a mixture that also recognizes Smcx (MGI:99781). This assay was designed to clarify that expression of the Y homolog (Smcy) in males did not eliminate the male/female dosage imbalance of the X homolog (Smcx) shown in Figure 5. Graph in Figure 6B displays summed expression of the X and Y homolog pair quantified from this northern blot in males vs. females. The probe used in this assay was 5' labelled.
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Results
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Image: 6A. Smcx/y
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Male |
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Male |
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Male |
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Male |
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Female |
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Female |
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Female |
| P adult
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TS28: brain
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Present
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Present
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4 µg; poly-A+ RNA |
C57BL/6J |
| Female |
Notes:
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Analysis Tools
