Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:81328
Natale DR, et al., Rac-1 and IQGAP are potential regulators of E-cadherin-catenin interactions during murine preimplantation development. Mech Dev. 2002 Dec;119 Suppl 1:S21-6
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:2652274
|
|
Gene symbol:
|
Iqgap1
|
|
Gene name:
|
IQ motif containing GTPase activating protein 1
|
|
Antibody:
|
anti-IQPAC-1
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
At least five embryos at each stage were stained. Embryos were viewed using a confocal microscope.
|
|
|
Results
|
Specimen
2a:
postnatal
(more  )
Note: Mature oocyte.
Specimen
2a:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Female
|
|
Type:
|
optical section
|
|
Note:
|
Mature oocyte.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Graded |
2a
|
Staining was present in the cytoplasm but was also localized to regions adjacent to the cell membrane and was particularly evident around the maternal chromosomes.
|
Specimen
2b:
embryonic day 1.0
(more )
Note: Two-cell embryo.
Specimen
2b:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 1.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Two-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Graded |
2b
|
Staining was present in the cytoplasm. There was intense staining encircling the cell borders and margins; this was most intense near unopposed cell membrances, compared to cell membranes near adjacent cells.
|
Specimen
2c:
embryonic day 2.0
(more )
Note: Four-cell embryo.
Specimen
2c:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 2.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Four-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
Graded |
2c
|
Staining was present in the cytoplasm. There was intense staining encircling the cell borders and margins; this was most intense near unopposed cell membrances, compared to cell membranes near adjacent cells.
|
Specimen
2d:
embryonic day 2.5
(more )
Note: Compacted 8-cell embryo.
Specimen
2d:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Compacted 8-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
|
2d
|
Expression was predominantly cytoplasmic with some apical staining in membrane-associated regions.
|
Specimen
2e:
embryonic day 3.0
(more )
Note: Morula.
Specimen
2e:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Morula.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
|
2e
|
Expression was present in the cytoplasm, as well as the apical regions of the outer cells. The apical staining was diffuse, with some additional punctate patterns.
|
Specimen
2f:
embryonic day 3.0
(more )
Note: Morula.
Specimen
2f:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Morula.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
|
2f
|
Expression was present in the cytoplasm in a diffuse pattern.
|
Specimen
2g:
embryonic day 4.0
(more )
Note: Early blastocyst.
Specimen
2g:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 4.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Early blastocyst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: inner cell mass |
Present |
|
2g
|
Expression was present in the cytoplasm in a diffuse pattern.
|
| TS5: trophectoderm |
Present |
|
2g
|
Expression was present in the cytoplasm in a diffuse pattern.
|
Specimen
2h:
embryonic day 4.0
(more )
Note: Blastocyst.
Specimen
2h:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 4.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Blastocyst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: trophectoderm |
Present |
Spotted |
2h
|
Expression was in a punctate, vesicular pattern.
|
| TS5: inner cell mass |
Present |
Regionally restricted |
2h
|
There was apolar expression encircling each cell.
|
|
Analysis Tools