Gene Expression Data
Assay Details
Assay
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Reference:
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J:81328
Natale DR, et al., Rac-1 and IQGAP are potential regulators of E-cadherin-catenin interactions during murine preimplantation development. Mech Dev. 2002 Dec;119 Suppl 1:S21-6
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2652274
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Gene symbol:
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Iqgap1
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Gene name:
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IQ motif containing GTPase activating protein 1
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Antibody:
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anti-IQPAC-1
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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At least five embryos at each stage were stained. Embryos were viewed using a confocal microscope.
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Results
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Specimen
2a:
postnatal
(more )
Note: Mature oocyte.
Specimen
2a:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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optical section
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Note:
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Mature oocyte.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Graded |
2a
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Staining was present in the cytoplasm but was also localized to regions adjacent to the cell membrane and was particularly evident around the maternal chromosomes.
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Specimen
2b:
embryonic day 1.0
(more )
Note: Two-cell embryo.
Specimen
2b:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Two-cell embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
Graded |
2b
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Staining was present in the cytoplasm. There was intense staining encircling the cell borders and margins; this was most intense near unopposed cell membrances, compared to cell membranes near adjacent cells.
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Specimen
2c:
embryonic day 2.0
(more )
Note: Four-cell embryo.
Specimen
2c:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Four-cell embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Present |
Graded |
2c
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Staining was present in the cytoplasm. There was intense staining encircling the cell borders and margins; this was most intense near unopposed cell membrances, compared to cell membranes near adjacent cells.
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Specimen
2d:
embryonic day 2.5
(more )
Note: Compacted 8-cell embryo.
Specimen
2d:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Compacted 8-cell embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
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2d
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Expression was predominantly cytoplasmic with some apical staining in membrane-associated regions.
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Specimen
2e:
embryonic day 3.0
(more )
Note: Morula.
Specimen
2e:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Morula.
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
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2e
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Expression was present in the cytoplasm, as well as the apical regions of the outer cells. The apical staining was diffuse, with some additional punctate patterns.
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Specimen
2f:
embryonic day 3.0
(more )
Note: Morula.
Specimen
2f:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Morula.
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
|
2f
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Expression was present in the cytoplasm in a diffuse pattern.
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Specimen
2g:
embryonic day 4.0
(more )
Note: Early blastocyst.
Specimen
2g:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 4.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
|
optical section
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Note:
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Early blastocyst.
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Structure |
Level |
Pattern |
Image |
Note |
TS5: inner cell mass |
Present |
|
2g
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Expression was present in the cytoplasm in a diffuse pattern.
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TS5: trophectoderm |
Present |
|
2g
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Expression was present in the cytoplasm in a diffuse pattern.
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Specimen
2h:
embryonic day 4.0
(more )
Note: Blastocyst.
Specimen
2h:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 4.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
optical section
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Note:
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Blastocyst.
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Structure |
Level |
Pattern |
Image |
Note |
TS5: trophectoderm |
Present |
Spotted |
2h
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Expression was in a punctate, vesicular pattern.
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TS5: inner cell mass |
Present |
Regionally restricted |
2h
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There was apolar expression encircling each cell.
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