Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:36725
Kim KK, et al., Mouse RAD50 has limited epitopic homology to p53 and is expressed in the adult myocardium. J Biol Chem. 1996 Nov 15;271(46):29255-64
|
Assay type:
|
Northern blot
|
MGI Accession ID:
|
MGI:2655076
|
Gene symbol:
|
Rad50
|
Gene name:
|
RAD50 double strand break repair protein
|
Probe:
|
Rad50 cDNA1
|
Probe preparation:
|
Double stranded labelled with radioactivity DNA
|
Visualized with:
|
Autoradiography
|
Assay notes:
|
The probe was radiolabeled. Densitometry was used to determine the relative level (%) of the 5.3kb transcript in each tissue as compared to AT-2 cardiomyocytes on the same blot. The values were further normalized to controls to account for variations inloading.
|
|
Results
|
In vitro data: no data stored |
P adult
|
TS28: brain
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: gut
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: heart
|
Strong
|
Present
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: metanephros
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: liver
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: lung
|
Strong
|
Present
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: skeletal muscle
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: skin
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: spleen
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: aorta
|
Strong
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
P adult
|
TS28: testis
|
Very strong
|
Not Specified
|
Present
|
10 µg; total RNA |
C3HeB/FeJ |
| Male |
P adult
|
TS28: uterus
|
Not Specified
|
Not Specified
|
Not Specified
|
10 µg; total RNA |
C3HeB/FeJ |
| Female |
Notes:
|